pFloxin-IRES-eGFP vector (V006231)

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LOCUS       40924_20196        5274 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFloxin-IRES-eGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Singla V, Hunkapiller J.
  TITLE     Efficient engineering of novel alleles at gene trap loci in mouse 
            embryonic stem cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Singla V, Hunkapiller J.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2009) Biochemistry, UCSF, 1550 4th Street, San 
            Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 5274)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUL-2009) Biochemistry, UCSF, 1550 4th Street, San Francisco, CA
            94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..525
                     /label=b-actin
                     /note="b-actin"
                     /regulatory_class="promoter"
     regulatory      520..529
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(532..565)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          566..831
                     /note="En2"
     misc_feature    879..1453
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1506..2222
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2426..2560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2578..2625
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(2649..2665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2673..2689)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2697..2727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2742..2763)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3051..3639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3813..4670)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4671..4775)
                     /label=AmpR promoter
     primer_bind     5249..5265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"