pFila vector (V006251)

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LOCUS       40924_20071        6486 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pFila, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Bi Y, Zheng X, Shao C, Pan W, Jiang L, Ouyang H.
  TITLE     Construction and application of a built-in dual luciferase reporter 
            for microRNA functional analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Bi Y, Zheng X, Shao C, Pan W, Jiang L, Ouyang H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2010) College of Life Science, Wuhan University, 
            Bayi Road, Wuhan, Hubei 430072, P.R. China
REFERENCE   3  (bases 1 to 6486)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6486)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2010) College of Life Science, Wuhan University, Bayi Road, 
            Wuhan, Hubei 430072, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1966
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     misc_feature    1971..1976
                     /label=XbaI restriction site
                     /note="XbaI restriction site"
     misc_recomb     1995..2000
                     /label=ApaI restriction site
                     /note="ApaI restriction site"
     polyA_signal    2028..2149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2039..2240
                     /label=SV40 late polyadenylation signal (upstream)
                     /note="SV40 late polyadenylation signal (upstream)"
                     /regulatory_class="polyA_signal_sequence"
     promoter        2279..2475
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             2511..4160
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(4204..4325)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4695..4799
                     /label=AmpR promoter
     CDS             4800..5657
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5831..6419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"