pFastBac1-HM vector (V006297)

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LOCUS       40924_19741        4939 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pFastBac1-HM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Katagiri Y, Ingham KC.
  TITLE     Enhanced production of green fluorescent fusion proteins in a 
            baculovirus expression system by addition of secretion signal
  JOURNAL   BioTechniques 33 (1), 24-26 (2002)
  PUBMED    12139250
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Sarafanov A, Saenko E.
  TITLE     High-throughput optimization of protein expression in the 
            baculovirus system based on determination of relative expression 
            efficiency of viral stocks
  JOURNAL   Anal. Biochem. 328 (1), 98-100 (2004)
  PUBMED    15081917
REFERENCE   3  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Pechik IV, Radtke KP, Khrenov AV, 
            Ananyeva NM, Strickland DK, Saenko EL.
  TITLE     Identification of coagulation factor VIII A2 domain residues forming
            the binding epitope for low-density lipoprotein receptor-related 
            protein
  JOURNAL   Biochemistry 45 (6), 1829-1840 (2006)
  PUBMED    16460029
REFERENCE   4  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Andersen OM, Mikhailenko IA, Ananyeva 
            NM, Khrenov AV, Shima M, Strickland DK, Saenko EL.
  TITLE     Localization of the low-density lipoprotein receptor-related protein
            regions involved in binding to the A2 domain of coagulation factor 
            VIII
  JOURNAL   Thromb. Haemost. 98 (6), 1170-1181 (2007)
  PUBMED    18064310
REFERENCE   5  (bases 1 to 4939)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   6  (bases 1 to 4939)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 4939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2002"; volume: "33"; issue: "1"; pages: 
            "24-26"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2004"; volume: "328"; issue: "1"; pages: "98-100"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: 
            "Biochemistry"; date: "2006"; volume: "45"; issue: "6"; pages: 
            "1829-1840"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Thromb. 
            Haemost."; date: "2007"; volume: "98"; issue: "6"; pages: 
            "1170-1181"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2004) Biochemistry, American Red Cross, Holland Laboratory, 
            15601 Crabbs Branch Way, Rockville, MD 20855, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2511..2735)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             4063..4074
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     sig_peptide     4081..4143
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             4156..4173
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      4204..4213
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4208..4239
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             4246..4275
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     polyA_signal    4429..4563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4592..4757)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"