Basic Vector Information
- Vector Name:
- pEG202 (pLexA)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10166 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Golemis E, Gyuris J, Brent R.
- Promoter:
- ADH1(long)
pEG202 (pLexA) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEG202 (pLexA) vector Sequence
LOCUS 40924_17114 10166 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEG202 (pLexA), complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10166)
AUTHORS Golemis E, Gyuris J, Brent R.
TITLE Interaction trap/two-hybrid systems to identify interacting proteins
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10166)
AUTHORS Golemis E, Gyuris J, Brent R.
TITLE pLexA (pEG202) complete sequence
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 10166)
AUTHORS Golemis E, Gyuris J, Brent R.
TITLE Direct Submission
JOURNAL Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE 4 (bases 1 to 10166)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 10166)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle,
Palo Alto, CA 94303-4230, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
call (415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424-8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH. If you suspect there is an
error in this sequence, please contact CLONTECH's Technical Support
Department at (415) 424-8222 or (800) 662-2566, extension 3 or
E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..10166
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 807..1511
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
CDS 1538..2143
/label=LexA
/note="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
regulatory 2165..2174
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
terminator 2209..2396
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
rep_origin 3128..4470
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(6117..6773)
/label=HIS3
/note="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
promoter complement(6774..6961)
/label=HIS3 promoter
promoter 8026..8130
/label=AmpR promoter
CDS 8131..8988
/label=AmpR
/note="beta-lactamase"
rep_origin 9162..9750
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(9936..10076)
/label=bom
/note="basis of mobility region from pBR322"
This page is informational only.