pattPGGsaacoriT vector (V009396)

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LOCUS       40924_5059        3670 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattPGGsaacoriT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
  PUBMED    24473925
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10"; 
            pages: "4557-4570"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     protein_bind    409..442
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     RBS             542..550
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             556..1329
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_feature    1342..1583
                     /label=oriT
                     /note="oriT"
     misc_recomb     1609..1658
                     /label=P-GG
                     /note="P-GG"
     primer_bind     complement(1665..1684)
     rep_origin      complement(1923..2511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2685..3542)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3543..3647)
                     /label=AmpR promoter