pattBCCsHygoriT vector (V009399)

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LOCUS       V009399                 3907 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009399
VERSION     V009399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
   PUBMED   24473925
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10";
            pages: "4557-4570"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label="B-CC"
                     /note="B-CC"
     protein_bind    472..505
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     RBS             620..628
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             634..1629
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1642..1883
                     /label="oriT"
                     /note="oriT"
     primer_bind     complement(1902..1921)
     rep_origin      complement(2160..2748)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2922..3779)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3780..3884)
                     /label="AmpR promoter"