pattB-QF-hsp70 vector (V009401)

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LOCUS       V009401                10124 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009401
VERSION     V009401
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q system: a repressible binary system for transgene expression,
            lineage tracing, and mosaic analysis
  JOURNAL   Cell 141 (3), 536-548 (2010)
   PUBMED   20434990
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Luo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2010) Neuroscience, The Johns Hopkins School of
            Medicine, 855 N. Wolfe Street, Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 10124)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
            date: "2010"; volume: "141"; issue: "3"; pages: "536-548"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-APR-2010) Neuroscience, The Johns Hopkins School of Medicine,
            855 N. Wolfe Street, Baltimore, MD 21205, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label="mini-white"
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4874..7321
                     /gene="qa-1f"
                     /label="Transcription factor qa-1f"
                     /note="Transcription factor qa-1f from Neurospora crassa
                     (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
                     FGSC 987). Accession#: P11638"
     regulatory      7331..7587
                     /label="hsp70 terminator"
                     /note="hsp70 terminator"
                     /regulatory_class="terminator"
     protein_bind    7648..7717
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7936..7954)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7975..7991)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7999..8015
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8023..8053)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8068..8089)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8377..8965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9139..9996)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9997..10101)
                     /label="AmpR promoter"