Basic Vector Information
- Vector Name:
- pANY6
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4094 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu Y.
- Promoter:
- tet
pANY6 vector Vector Map
pANY6 vector Sequence
LOCUS 40924_4559 4094 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pANY6, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4094) AUTHORS An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu Y. TITLE Three novel Escherichia coli vectors for convenient and efficient molecular biological manipulations JOURNAL J. Agric. Food Chem. (2018) In press REFERENCE 2 (bases 1 to 4094) AUTHORS An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu Y. TITLE Direct Submission JOURNAL Submitted (22-MAY-2018) College of Biosciences and Biotechnology, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110161, P. R. China. REFERENCE 3 (bases 1 to 4094) TITLE Direct Submission REFERENCE 4 (bases 1 to 4094) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Agric. Food Chem. (2018) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-MAY-2018) College of Biosciences and Biotechnology, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110161, P. R. China." COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4094 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 53..641 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 718..736 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 768..790 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" misc_feature 798..1784 /label=ELP tag /note="ELP tag" misc_feature 1785..2294 /label=intein /note="intein" regulatory 2295..2326 /label=multiple cloning sites (MCS)-1 /note="multiple cloning sites (MCS)-1" /regulatory_class="other" CDS 2657..2959 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" regulatory 2989..3026 /label=multiple cloning sites (MCS)-2 /note="multiple cloning sites (MCS)-2" /regulatory_class="other" terminator 3054..3101 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter complement(3169..3197) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS complement(3213..4025) /label=KanR /note="aminoglycoside phosphotransferase"
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