pCAG.I-SceI vector (V008887)

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LOCUS       V008887                 5811 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008887
VERSION     V008887
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Holkers M, de Vries AA, Goncalves MA.
  TITLE     Nonspaced inverted DNA repeats are preferential targets for
            homology-directed gene repair in mammalian cells
  JOURNAL   Nucleic Acids Res. 40 (5), 1984-1999 (2012)
   PUBMED   22080552
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Holkers M, de Vries AF, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2011) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 5811)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5811)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "5"; pages:
            "1984-1999"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2011) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..658
                     /label="chicken beta-actin promoter"
     intron          659..1667
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1762..1782
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1804..1824
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1843..1869
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1873..2577
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     polyA_signal    2742..2797
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3159..3175)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3183..3199)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3207..3237)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3252..3273)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3561..4149)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4323..5180)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5181..5285)
                     /label="AmpR promoter"
     primer_bind     5759..5775
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5809..5811
                     /label="CAG"
                     /note="CMV early enhancer fused to modified chicken
                     beta-actin promoter"