pBrachyTAG-G229 vector (V009096)

LOCUS       V009096                 9085 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009096
VERSION     V009096
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Thole V, Worland B, Vain P.
  TITLE     pBrachyTAG vector system: New tools for DNA tagging and trapping in
            grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2010) Crop Genetics, John Innes Centre, Norwich
            Research Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 9085)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-2010) Crop Genetics, John Innes Centre, Norwich Research
            Park, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     spacer sequence from left border No.1"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic unique sequence containing
                     forward and reverse primer sequences"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4672..4964)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     regulatory      complement(4974..4985)
                     /gene="sgfp(S65T)-ER"
                     /note="C-terminal HDEL signal sequence for protein
                     secretion and retention in the ER"
                     /regulatory_class="other"
     CDS             complement(4986..5699)
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     regulatory      complement(5700..5765)
                     /gene="sgfp(S65T)-ER"
                     /note="N-terminal ER signal peptide sequence from
                     Arabidopsis thaliana basic chitinase"
                     /regulatory_class="other"
     promoter        complement(5774..7770)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    7820..7923
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    7930..7954
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    7955..7978
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             8182..8982
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"