Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V001363 | pET-28a-EGFP-C | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
E.coli expression vector with a Green Fluorescent Protein.
- Vector Name:
- pET-28a-EGFP-C
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6088 bp
- Type:
- E. coli Expression Vectors, Fluorescent Protein Re
- Replication origin:
- ori
- Selection Marker:
- C-EGFP
- Promoter:
- T7/lac
- Growth Strain(s):
- TOP10
- Growth Temperature:
- 37℃
pET-28a-EGFP-C vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- He Z, Chen K, An Y, He J, Zhang X, Tang L, Sun F, Jiang K. BSA modification of bacterial surface: a promising anti-cancer therapeutic strategy. BMC Microbiol. 2023 Apr 17;23(1):105.
pET-28a-EGFP-C vector Sequence
LOCUS 40924_17811 6088 bp DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6088)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6088)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6088
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(563..1375)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 1497..2085
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2271..2413)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(2518..2706)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(3481..3502)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3518..4597)
/label=lacI
/note="lac repressor"
promoter complement(4598..4675)
/label=lacI promoter
promoter 4984..5002
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5003..5027
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5042..5064
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5083..5100
/label=6xHis
/note="6xHis affinity tag"
CDS 5110..5127
/label=thrombin site
/note="thrombin recognition and cleavage site"
CDS 5131..5163
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=leader peptide from bacteriophage T7 gene 10
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 5206..5922
/label=EGFP
/note="enhanced GFP"
CDS 5932..5949
/label=6xHis
/note="6xHis affinity tag"
terminator 6016..6063
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"