pZR80 vector (V001826)

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LOCUS       V001826                 6405 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001826
VERSION     V001826
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Kok RG, Young DM, Ornston LN.
  TITLE     Phenotypic expression of PCR-generated random mutations in a
            Pseudomonas putida gene after its introduction into an Acinetobacter
            chromosome by natural transformation
  JOURNAL   Appl. Environ. Microbiol. 65 (4), 1675-1680 (1999)
   PUBMED   10103267
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Young DM, Kok RG, Ornston L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Molecular, Cellular, and Developmental
            Biology, Yale University, P.O. Box 208103 Room KBT 840, New Haven,
            CT 06520, USA
REFERENCE   3  (bases 1 to 6405)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "1999"; volume: "65"; issue: "4"; pages:
            "1675-1680"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUN-2000) Molecular, Cellular, and Developmental Biology, Yale
            University, P.O. Box 208103 Room KBT 840, New Haven, CT 06520, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             571..1599
                     /gene="lifO"
                     /label="Lipase chaperone"
                     /note="Lipase chaperone from Acinetobacter baylyi (strain
                     ATCC 33305 / BD413 / ADP1). Accession#: Q43961"
     promoter        1749..1777
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    1929..1985
                     /label="polylinker"
                     /note="polylinker"
     regulatory      2007..2012
                     /label="PaphA3"
                     /note="PaphA3"
                     /regulatory_class="promoter"
     CDS             2069..2860
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2929..3096
                     /label="truncated lipA"
                     /note="truncated lipA"
     rep_origin      complement(4568..5156)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5330..6187)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6188..6292)
                     /label="AmpR promoter"