pZNR0943 vector (V001830)

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LOCUS       40924_48538        7416 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZNR0943, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in 
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
  PUBMED    25984720
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber 
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA
REFERENCE   3  (bases 1 to 7416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..17
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    19..22
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     regulatory      189..888
                     /regulatory_class="promoter"
     misc_feature    complement(894..897)
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     misc_feature    complement(899..904)
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     regulatory      905..1070
                     /regulatory_class="promoter"
     RBS             1053..1064
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1071..1784
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    1788..1793
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     misc_feature    1795..1798
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     CDS             1799..2491
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      2501..2688
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(2895..2898)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    complement(2900..2905)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    2917..3420
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     regulatory      complement(3426..3902)
                     /regulatory_class="terminator"
     CDS             complement(3906..4997)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4998..5405)
                     /label=LEU2 promoter
     rep_origin      complement(5761..6349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6426..6573
                     /regulatory_class="promoter"
     CDS             6574..7386
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      7390..7416
                     /regulatory_class="terminator"