pZJY156 vector (V001837)

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LOCUS       40924_48498        5933 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pZJY156, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Zhao JY, Zhong L, Shen MJ, Xia ZJ, Cheng QX, Sun X, Zhao GP, Li YZ, 
            Qin ZJ.
  TITLE     Discovery of the autonomously replicating plasmid pMF1 from 
            Myxococcus fulvus and development of a gene cloning system in 
            Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 1980-1987 (2008)
  PUBMED    18245244
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Zhao J, Zhong L, Qin Z, Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) State Key Laboratory of Microbial 
            Technology, School of Life Science, Shandong University, 27 South 
            Road of Shanda, Jinan, Shandong 250100, People's Republic of China
REFERENCE   3  (bases 1 to 5933)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "7"; pages:
            "1980-1987"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2007) State Key Laboratory of Microbial Technology, School 
            of Life Science, Shandong University, 27 South Road of Shanda, 
            Jinan, Shandong 250100, People's Republic of China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             299..1087
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1429..3333
                     /codon_start=1
                     /gene="rep-pMF1"
                     /product="putative Rep-pMF1 protein"
                     /label=rep-pMF1
                     /protein_id="ABY83642.1"
                     /translation="MKRRALTSQPTPARALVHGAGSGERPTLREPSRAKSQPRNRRSGL
                     DVEPSAASASSLPADQVAQLRASTARLRREQSQRQRRVNAGKARRNEERKWAALELATG
                     TALPLVDTVGFRPAEVSKPETYVTLEDTHGLEGLARGERAEQELEAFRASHPSRQVAPP
                     AALAIAAILYSTRKAEGGADARDCASLGWQVPVRGWCEMTGYRERAVQAAFAWLADEGL
                     VRRFGDYVTVKHLTRLRPAGERLDLWLDAKGKARRWVQVYSVTYLTNAGAAWLERAGFD
                     LAETRGHGVAWRRRGFLARVGRALAGLWRTVRRRLGGGGSVAVGAGATCTPYPAKQVEV
                     CSRRGDDSQPTASLEELIGAESPPHGGDVTGSASAKPDPRAANAEHARVEGAPAQGGHA
                     SMGSASAKLEGSKPPPSALGPEWEKKLGLTAEGIAAGLAWEGGGLLAYAEVVDRCWTRT
                     TTTTRAAPSDVPSGSRNMFGRRRVWRCRGCGAESRYKGATVRHAQGCTAVGAWHLARGP
                     AVETWSPAMTAELAWPEDWEAHPELRPRLRRLFVPVAAELTRRIQARAAEARRAAEAEE
                     AERRHLEVWMSKHPECGGAFRAPVYDSSVCVADGPPNAWGRAPLCYCDACSQKRRDIE"
     gene            1429..3333
                     /gene="rep-pMF1"
                     /label=rep-pMF1
     promoter        complement(3571..3589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3847..4435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4609..5466)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5467..5571)
                     /label=AmpR promoter
     promoter        5917..5933
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"