pZJ25 vector (V001838)

LOCUS       V001838                 5231 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001838
VERSION     V001838
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.
  TITLE     Physiological roles of pyruvate ferredoxin oxidoreductase and
            pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum
            JW/SL-YS485
  JOURNAL   Biotechnol Biofuels 8, 138 (2015)
   PUBMED   26379770
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Zhou J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2014) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5231)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 8, 138 (2015)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-OCT-2014) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..741
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_feature    802..1704
                     /label="upstream of Tsac_0628"
                     /note="upstream of Tsac_0628"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2709..3566)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3567..3671)
                     /label="AmpR promoter"
     misc_feature    3765..4680
                     /label="downstream of Tsac_0629"
                     /note="downstream of Tsac_0629"
     regulatory      4688..5231
                     /label="kanamycin promoter from pMU433"
                     /note="kanamycin promoter from pMU433"
                     /regulatory_class="promoter"