Price Information
| Cat No. | Plasmid Name | Availability | Buy one, get one free! (?) |
|---|---|---|---|
| V001858 | pZA3PLtetO-1 luc | In stock, instant shipping |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
pZA3PLtetO-1 luc is a tetracycline-inducible plasmid containing the pLtetO-1 promoter and luciferase gene. It activates luc expression upon addition of tetracycline/Dox, enabling quantitative detection of dynamic changes in gene regulation or drug induction.
- Vector Name:
- pZA3PLtetO-1 luc
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 3666 bp
- Type:
- Expression vector
- Replication origin:
- p15A ori
- Source/Author:
- Lutz R, Bujard H.
- Growth Strain(s):
- Stbl3
pZA3PLtetO-1 luc vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pZA3PLtetO-1 luc vector Sequence
LOCUS Exported 3666 bp DNA circular SYN 31-OCT-2025
DEFINITION Expression vector pZA3PLtetO-1 luc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3666)
AUTHORS Lutz R, Bujard H.
TITLE Independent and tight regulation of transcriptional units in
Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2
regulatory elements
JOURNAL Nucleic Acids Res. 25 (6), 1203-1210 (1997)
PUBMED 9092630
REFERENCE 2 (bases 1 to 3666)
AUTHORS Lutz R, Bujard H.
TITLE Direct Submission
JOURNAL Submitted (07-AUG-1996) Zentrum fuer Molekulare Biologie, University
of Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany
REFERENCE 3 (bases 1 to 3666)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3666)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 3666)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "1997"; volume: "25"; issue: "6"; pages:
"1203-1210"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-AUG-1996) Zentrum fuer Molekulare Biologie, University of
Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3666
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 7..80
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
RBS 90..101
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 108..1757
/label=luciferase
/note="firefly luciferase"
terminator 1776..1862
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2032..2577)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
terminator complement(2691..2785)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(2809..3465)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(3466..3568)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"