pYMD1 vector (V001874)

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LOCUS       40924_48218        4641 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pYMD1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Binding sequences for RdgB, a DNA damage-responsive transcriptional 
            activator, and temperature-dependent expression of bacteriocin and 
            pectin lyase genes in Pectobacterium carotovorum subsp. carotovorum
  JOURNAL   Appl. Environ. Microbiol. 74 (19), 6017-6025 (2008)
  PUBMED    18689515
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Yamada K, Kaneko J, Kamio Y, Itoh Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2007) Contact:Kazuteru Yamada Graduate School of 
            Agricultural Science, Tohoku University, Laboratory of Applied 
            Microbiology, Department of Microbial Biotechnology; 1-1 
            Tsutsumi-dori Amaiya-machi, Aobaku, Sendai 981-8555, Japan
REFERENCE   3  (bases 1 to 4641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "19"; 
            pages: "6017-6025"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2007) Contact:Kazuteru Yamada Graduate School of 
            Agricultural Science, Tohoku University, Laboratory of Applied 
            Microbiology, Department of Microbial Biotechnology; 1-1 
            Tsutsumi-dori Amaiya-machi, Aobaku, Sendai 981-8555, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        110..555
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             556..1974
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(2708..2724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2760..3143)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3652..4509)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4510..4614)
                     /label=AmpR promoter