pYM70 vector (V001875)

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LOCUS       40924_48213        7331 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYM70, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Gast CE, Mao Y, Wong B.
  TITLE     Fluconazole transport into Candida albicans secretory vesicles by 
            the membrane proteins Cdr1p, Cdr2p, and Mdr1p
  JOURNAL   Eukaryotic Cell 9 (6), 960-970 (2010)
  PUBMED    20348384
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Bartiss A, Mao Y, Gast CE, Coelho PS, Snyder M, Wong 
            B.
  TITLE     Transformation of Candida albicans with a synthetic hygromycin B 
            resistance gene
  JOURNAL   Yeast 27 (12), 1039-1048 (2010)
  PUBMED    20737428
REFERENCE   3  (bases 1 to 7331)
  AUTHORS   Mao Y, Basso LR Jr., Gast CE, Wong B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2010) Medicine, Oregon Health and Science 
            University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
REFERENCE   4  (bases 1 to 7331)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7331)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2010"; volume: "9"; issue: "6"; pages: "960-970"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Yeast"; 
            date: "2010"; volume: "27"; issue: "12"; pages: "1039-1048"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-FEB-2010) Medicine, Oregon Health and Science University, 3181 
            SW Sam Jackson Park Road, Portland, OR 97239, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      189..1390
                     /label=Candida albicans autonomous replication origin 2
                     /note="Candida albicans autonomous replication origin 2"
     primer_bind     1587..1603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1637..2652
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     regulatory      2684..3009
                     /label=Candida albicans TEF2 terminator
                     /note="Candida albicans TEF2 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3037..3053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3061..3077)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3085..3115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3130..3151)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3439..4027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4201..5058)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5059..5163)
                     /label=AmpR promoter
     regulatory      5195..5835
                     /label=Candida albicans TEF2 promoter
                     /note="Candida albicans TEF2 promoter"
                     /regulatory_class="promoter"
     CDS             5842..6864
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      6874..7261
                     /label=Candida albicans ACT1 terminator
                     /note="Candida albicans ACT1 terminator"
                     /regulatory_class="terminator"