pFastBacMam5 vector (V016014)

Download GeneBank File(.gb)

LOCUS       62056_10790        7446 bp DNA     circular SYN 10-NOV-2021
DEFINITION  Synthetic construct DNA, expression vector pFastBacMam5, complete 
            sequence.
ACCESSION   LC658352
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Vectors for baculovirus gene transfer into Mammalian cells to 
            product recombinant Flag-His8-eGFP-3C fusion proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, 
            Cellular and Structural Physiology Institute; Furo-cho, Chikusa-ku, 
            Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, Cellular 
            and Structural Physiology Institute; Furo-cho, Chikusa-ku, Nagoya, 
            Aichi 4648601, Japan"
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    complement(377..511)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     536..557
                     /label=pFastBac primer R1
                     /note="pFastBac primer R1"
     enhancer        797..1175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1176..1379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1376..1400
                     /label=LNCX_primer
                     /note="LNCX_primer"
     intron          1515..1647
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1692..1710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1730..1753
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1775..1798
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             1799..2515
                     /label=mEGFP
                     /note="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             2525..2548
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    2594..2596
                     /label=stop codon
                     /note="stop codon"
     misc_feature    2597..3185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(3197..3318)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3452..3543)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3895..3923
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4112..4642
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     mobile_element  4712..4936
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5239..5827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6001..6858)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6859..6963)
                     /label=AmpR promoter
     rep_origin      complement(6990..7445)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"