pVG344_Aste_kh2-MCRv3-Vasa-Cas9_SWAP vector (V015953)

LOCUS       62056_22565        7040 bp DNA     circular SYN 07-OCT-2020
DEFINITION  Cloning vector pVG344_Aste_kh2-MCRv3-Vasa-Cas9_SWAP, complete 
            sequence.
ACCESSION   MW030448
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Efficient population modification gene-drive rescue system in the 
            malaria mosquito Anopheles stephensi
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Developmental and Stem Cell Biology, 
            University of California, San Francisco, 1650 Owens St., San 
            Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 7040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2020) Developmental and Stem Cell Biology, University of 
            California, San Francisco, 1650 Owens St., San Francisco, CA 94158, 
            USA"
FEATURES             Location/Qualifiers
     source          1..7040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(174..1031)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     misc_feature    1299..3187
                     /label=kynurenine hydroxylase
                     /note="kynurenine hydroxylase"
     misc_feature    2615..3090
                     /label=recoded kynurenine hydroxylase
                     /note="recoded kynurenine hydroxylase"
     misc_feature    3091..3290
                     /label=Anopheles gambiae genomic fragment
                     /note="Anopheles gambiae genomic fragment"
     3'UTR           3091..3187
                     /label=kynurenine hydroxylase 3'UTR
                     /note="kynurenine hydroxylase 3'UTR"
     misc_feature    3291..3340
                     /label=attP
                     /note="attP"
     polyA_signal    complement(3362..3483)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3594..3610
                     /label=gRNA SWAP1
                     /note="gRNA SWAP1"
     CDS             complement(3616..4332)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      complement(4381..4603)
                     /label=3xP3 promoter
                     /note="3xP3 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(4645..6327)
                     /label=Vasa promoter
                     /note="Vasa promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(6452..7040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"