pFruR vector (V015541)

LOCUS       V015541                 3852 bp    DNA     circular SYN 10-SEP-2020
DEFINITION  Exported.
ACCESSION   V015541
VERSION     V015541
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
   PUBMED   32895374
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-FEB-2020) Chemical "
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             674..1675
                     /gene="cra"
                     /label="Catabolite repressor/activator"
                     /note="Catabolite repressor/activator from Escherichia coli
                     (strain K12). Accession#: P0ACP1"
     protein_bind    1808..1829
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      2020..2564
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        3090..3192
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3193..3849
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"