pANe-2 vector (V015471)

LOCUS       62056_3120        2648 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pANe-2, complete sequence.
ACCESSION   MN436793
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(44..49)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    51..54
                     /label=B
                     /note="B"
     misc_feature    56..58
                     /label=Alpha
                     /note="Alpha"
     misc_feature    complement(60..66)
                     /label=SapI
                     /note="SapI"
     protein_bind    130..151
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        166..196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(257..313)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(314..330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    665..671
                     /label=SapI
                     /note="SapI"
     misc_feature    673..675
                     /label=Omega
                     /note="Omega"
     misc_feature    677..680
                     /label=C
                     /note="C"
     misc_feature    682..687
                     /label=BsaI
                     /note="BsaI"
     misc_feature    691..730
                     /label=UNSX
                     /note="UNSX"
     misc_feature    1120
                     /label=Mut T->C
                     /note="Mut T->C"
     rep_origin      complement(1177..1722)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(1817..2473)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2474..2577)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"