pKS300IC vector (V005188)

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LOCUS       V005188                 9266 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005188
VERSION     V005188
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Cloning vector pKS300IC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2016) Contact:Asao Fujiyama National Instetute of
            Genetics, Center for Information Biology; 1111 Yata, Mishima,
            Shizuoka 411-8540, Japan URL :http://www.nig.ac.jp/
REFERENCE   3  (bases 1 to 9266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2016) Contact:Asao Fujiyama National Instetute of Genetics,
            Center for Information Biology; 1111 Yata, Mishima, Shizuoka
            411-8540, Japan URL :http://www.nig.ac.jp/"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..108)
                     /label="pKS300IC_Rev"
                     /note="pKS300IC_Rev"
     promoter        160..264
                     /label="AmpR promoter"
     CDS             265..1122
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(1610..2083)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2159..3127)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(3130..4302)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(4628..4878)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4884..5636)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(5727..5946)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             complement(6413..7216)
                     /gene="resD"
                     /label="Resolvase"
                     /note="Resolvase from Escherichia coli (strain K12).
                     Accession#: P06615"
     CDS             complement(7220..7522)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7527..7742)
                     /gene="ccdA"
                     /label="Antitoxin CcdA"
                     /note="Antitoxin CcdA from Escherichia coli (strain K12).
                     Accession#: P62552"
     primer_bind     8097..8117
                     /label="pKS300IC_Fwd"
                     /note="pKS300IC_Fwd"