pHomeD-B2-SCE-R vector (V005527)

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LOCUS       V005527                11424 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005527
VERSION     V005527
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Windbichler N, Menichelli M, Papathanos PA, Thyme SB, Li H, Ulge UY,
            Hovde BT, Baker D, Monnat RJ Jr., Burt A, Crisanti A.
  TITLE     A synthetic homing endonuclease-based gene drive system in the human
            malaria mosquito
  JOURNAL   Nature 473 (7346), 212-215 (2011)
   PUBMED   21508956
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Windbichler N.
  TITLE     Homing
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11424)
  AUTHORS   Windbichler N.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Imperial College London, Imperial College
            Road, London SW7 2AZ, England
REFERENCE   4  (bases 1 to 11424)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
            date: "2011"; volume: "473"; issue: "7346"; pages: "212-215"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-AUG-2010) Imperial College London, Imperial College Road, London
            SW7 2AZ, England"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(18..440)
                     /gene="beta-2-tubulin"
                     /regulatory_class="terminator"
     gene            complement(18..440)
                     /gene="beta-2-tubulin"
                     /label="beta-2-tubulin"
     CDS             complement(459..1163)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             complement(1167..1193)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(1212..1232)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     regulatory      complement(1289..1880)
                     /gene="beta-2-tubulin"
                     /regulatory_class="promoter"
     gene            complement(1289..1880)
                     /gene="beta-2-tubulin"
                     /label="beta-2-tubulin"
     CDS             1903..2619
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2741..2862
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2930..5507
                     /label="Ac5 promoter"
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             5519..6196
                     /label="DsRed1"
                     /note="wild-type DsRed"
     polyA_signal    6367..6797
                     /label="hsp70 poly(A)"
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(7917..7986)
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     primer_bind     complement(8022..8038)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8046..8062
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8070..8100)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8115..8136)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8424..9012)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9186..10043)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10044..10148)
                     /label="AmpR promoter"
     regulatory      11124..11385
                     /gene="3xP3"
                     /regulatory_class="promoter"
     gene            11124..11385
                     /gene="3xP3"
                     /label="3xP3"