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Product Name
Mouse BCHE/Butyrylcholinesterase (His Tag) recombinant protein
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Description
Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.
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Protein name
Cholinesterase
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Protein short names
ACYLCHOLINE ACYLHYDROLASE; PSEUDOCHOLINESTERASE; C730038G20RIK; BUTYRYLCHOLINE ESTERASE; CHE1; CHE2; BUTYRYLCHOLINESTERASE; BCHE; MGC107651; CHOLINE ESTERASE II; CHOLINESTERASE 1; E1; CHOLINESTERASE
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Uniprot ID
Q03311
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Gene Name
Bche
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse BCHE (NP_033868.3) (Met 1-Leu 603) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse BCHE consists of 587 amino acids and has a calculated molecular mass of 66.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 70 nmol/min/μg.
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Validations
Mouse BCHE / Butyrylcholinesterase Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"