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Product Name
PLK4 antibody
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Description
PLK4 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human cervical cancer tissue, human lung cancer tissue. Positive WB detected in mouse lung tissue, A549 cells, MCF7 cells. Observed molecular weight by Western-blot: 105-112 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PLK 4 antibody; PLK4 antibody; Polo like kinase 4 antibody; SAK antibody; STK18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLK4 recombinant protein (Accession Number: NM_014264). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:113971(PLK4 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human testis using Catalog No:113971(PLK4 antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113971(PLK4 antibody) at dilution of 1:200 (under 40x lens)
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Background
PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.
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References
- Fan G, Sun L, Shan P. Loss of KLF14 triggers centrosome amplification and tumorigenesis. Nature communications. 6:8450. 2015.
- Harris RM, Weiss J, Jameson JL. Male hypogonadism and germ cell loss caused by a mutation in Polo-like kinase 4. Endocrinology. 152(10):3975-85. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"