pCK53 vector (V008490)

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LOCUS       40924_10846        4867 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCK53, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Kemmer C, Fluri DA, Witschi U, Passeraub A, Gutzwiller A, 
            Fussenegger M.
  TITLE     A designer network coordinating bovine artificial insemination by 
            ovulation-triggered release of implanted sperms
  JOURNAL   J Control Release 150 (1), 23-29 (2011)
  PUBMED    21108977
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Kemmer C, Fluri DA, Witschi U, Passeraub A, Gutzwiller A, 
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, 
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 4867)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4867)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Control 
            Release"; date: "2011"; volume: "150"; issue: "1"; pages: "23-29"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, 
            Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..1505
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(1563..1684)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2103..2691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2865..3722)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3723..3827)
                     /label=AmpR promoter
     rep_origin      3854..4309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4440..4488
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4502..4593
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     regulatory      4627..4866
                     /label=CRE promoter
                     /note="CRE promoter"
                     /regulatory_class="promoter"