pmScarlet3-N1 vector (V012810#)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012810 pmScarlet3-N1 In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

mScarlet3 is a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems. [ref: Gadella, T.W.J., van Weeren, L., Stouthamer, J. et al. mScarlet3: a brilliant and fast-maturing red fluorescent protein. Nat Methods (2023).]

      • Vector Name:
      • pmScarlet3-N1
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 4703 bp
      • Type:
      • Mammalian Expression Vectors
      • Copy Number:
      • High copy number
      • Promoter:
      • CMV
      • Cloning Method:
      • Enzyme Cut
      • Fusion Tag:
      • mScarlet3
      • Expression Method:
      • Transient

pmScarlet3-N1 vector Vector Map

pmScarlet3-N14703 bp600120018002400300036004200CMV enhancerCMV promoterMCSmScarlet3SV40 poly(A) signalf1 oriAmpR promoterSV40 promoteraph(3')-II (or nptII)HSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pmScarlet3-N1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4703 bp ds-DNA     circular SYN 29-MAR-2023
DEFINITION  Vector for fusing EGFP to the C-terminus of a partner protein. For 
            other reading frames, use pEGFP-N2 or pEGFP-N3.
KEYWORDS    pmScarlet3-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4703)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        61..364
                     /note="CMV enhancer"
                     /note="CMV enhancer;human cytomegalovirus immediate early 
     promoter        365..568
                     /note="CMV promoter"
                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 
                     early promoter"
     misc_feature    591..671
                     /note="MCS;multiple cloning site of fluorescent protein 
     CDS             679..1368
     polyA_signal    1491..1612
                     /note="SV40 poly(A) signal"
                     /note="SV40 poly(A) signal;SV40 polyadenylation signal"
     rep_origin      complement(1619..2074)
                     /note="f1 ori"
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2101..2205
                     /note="bla AmpR promoter"
                     /note="AmpR promoter"
     promoter        2207..2564
                     /note="SV40 promoter"
                     /note="SV40 promoter;SV40 enhancer and early promoter"
     rep_origin      2415..2550
                     /note="SV40 ori"
                     /note="SV40 ori;SV40 origin of replication"
     CDS             2599..3393
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="aph(3')-II (or nptII)"
                     /note="NeoR/KanR;confers resistance to neomycin, kanamycin,
                     and G418 (Geneticin)"
     misc_feature    3625..3672
                     /note="HSV TK poly(A) signal"
                     /note="HSV TK poly(A) signal;herpesvirus thymidine kinase 
                     polyadenylation signal"
     rep_origin      4001..4589
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of