Basic Vector Information
- Vector Name:
- pcDNA3.1(+)-2C11-CD8TM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6361 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pcDNA3.1(+)-2C11-CD8TM vector Map
pcDNA3.1(+)-2C11-CD8TM vector Sequence
LOCUS . 6361 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 202..581
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 582..785
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 830..848
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1928..2152
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 2198..2626
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2640..2969
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3036..3827
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 4004..4137
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(4174..4190)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4198..4214)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(4222..4252)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4267..4288)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4576..5161)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5335..6192)
/label="AmpR"
/note="β-lactamase"
promoter complement(6193..6297)
/label="AmpR promoter"
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