LOCUS       V004403                 6196 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004403
VERSION     V004403
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A.
  TITLE     Tolerated wobble mutations in siRNAs decrease specificity, but can
            enhance activity in vivo
  JOURNAL   Nucleic Acids Res. 33 (15), 4704-4710 (2005)
   PUBMED   16113241
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Holen T, Ottersen OP, Klungland A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo 0317, Norway
REFERENCE   3  (bases 1 to 6196)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2005"; volume: "33"; issue: "15"; pages:
            "4704-4710"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo
            0317, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             248..1216
                     /gene="Aqp4"
                     /label="Aquaporin-4"
                     /note="Aquaporin-4 from Mus musculus. Accession#: P55088"
     CDS             1220..2869
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(2913..3034)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3699..4287)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4461..5318)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5319..5423)
                     /label="AmpR promoter"
     rep_origin      5450..5905
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6036..6084
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6098..6189
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt agtgacagag ctgcggcaag gcggtggggt aagtgtggac attcctgcag
      301 tagagagagc atcatggtgg ctttcaaagg agtctggact caggctttct ggaaggcagt
      361 ctcagcagaa tttctggcca cgcttatctt tgttttgctc ggtgtgggat ccaccataaa
      421 ctggggtggc tcagaaaacc ccttacctgt ggacatggtc ctcatctccc tttgctttgg
      481 actcagcatt gctaccatgg tgcagtgctt tggccacatc agtggtggcc acatcaatcc
      541 cgctgtgact gtagccatgg tgtgcacacg aaagatcagc atcgctaagt ccgtcttcta
      601 catcattgca cagtgcctgg gggccatcat tggagccggc atcctctacc tggtcacacc
      661 tcccagtgtg gttggaggat tgggagtcac cacggttcat ggaaacctca ccgctggcca
      721 tgggctcctg gtggagttaa taatcacttt ccagttggtg ttcactattt ttgccagctg
      781 tgattccaaa cgaactgatg ttactggttc aatagcttta gcaattggat tttccgttgc
      841 aattggacat ttgtttgcaa tcaattatac tggagccagc atgaatccag ctcgatcttt
      901 tggacccgca gttatcatgg gaaactgggc aaaccactgg atatattggg ttggaccaat
      961 catgggcgct gtgctggcag gtgcccttta tgagtatgtc ttctgtcctg atgtggagct
     1021 caaacgtcgc cttaaggaag ccttcagcaa agccgcgcag cagacaaaag ggagctacat
     1081 ggaggtggag gacaaccgga gccaagtgga gacggaagac ttgatcctga agcccggagt
     1141 ggtgcatgtg attgacattg accgtggaga agagaagaag gggaaagact cttcgggaga
     1201 ggtattgtct tccgtactca tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     1261 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     1321 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
     1381 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     1441 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     1501 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     1561 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
     1621 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
     1681 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     1741 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
     1801 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
     1861 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     1921 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     1981 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
     2041 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
     2101 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     2161 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     2221 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     2281 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     2341 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     2401 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     2461 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     2521 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     2581 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     2641 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     2701 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     2761 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     2821 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagag
     2881 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2941 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3001 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3061 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     3121 tggtaaaatc gataaggatc tgaacgatgg agcggagaat gggcggaact gggcggagtt
     3181 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     3241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     3301 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     3361 aactgacaca cattccacag cggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     3421 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3481 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     3541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5821 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5881 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa
     5941 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     6001 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     6061 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     6121 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     6181 catttctcta tcgata
//
