LOCUS       40924_24732        4707 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Monster GFP vector phMGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Sundquist T, Chauvin F, Almond B.
  TITLE     Monster Green Fluorescent Protein phMGFP Vector Technical Bulletin, 
            TB320
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Sundquist T, Chauvin F, Almond B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4707)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1076..1753
                     /codon_start=1
                     /label=mc4
                     /note="mc4 is a basic (constitutively fluorescent)
                     green/yellow fluorescent protein published in 2003, derived
                     from Montastraea cavernosa."
                     /translation="MGVIKPDMKIKLRMEGAVNGHKFVIEGDGKGKPFEGKQTMDLTVI
                     EGAPLPFAYDILTTVFDYGNRVFAKYPKDIPDYFKQTFPEGYSWERSMTYEDQGICIAT
                     NDITMMKGVDDCFVYKIRFDGVNFPANGPVMQRKTLKWEPSTEKMYVRDGVLKGDVNMA
                     LLLEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHRIEIVSHDKDYNKVKLYEHAEAHSGL
                     PRQA"
     promoter        complement(1781..1798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1816..1937)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2123..2578
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2910..3014
                     /label=AmpR promoter
     CDS             3015..3872
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4046..4634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcccc ggggatatcg ccaccatggg
     1081 cgtgatcaag cccgacatga agatcaagct gcggatggag ggcgccgtga acggccacaa
     1141 attcgtgatc gagggcgacg ggaaaggcaa gccctttgag ggtaagcaga ctatggacct
     1201 gaccgtgatc gagggcgccc ccctgccctt cgcttatgac attctcacca ccgtgttcga
     1261 ctacggtaac cgtgtcttcg ccaagtaccc caaggacatc cctgactact tcaagcagac
     1321 cttccccgag ggctactcgt gggagcgaag catgacatac gaggaccagg gaatctgtat
     1381 cgctacaaac gacatcacca tgatgaaggg tgtggacgac tgcttcgtgt acaaaatccg
     1441 cttcgacggg gtcaacttcc ctgctaatgg cccggtgatg cagcgcaaga ccctaaagtg
     1501 ggagcccagt accgagaaga tgtacgtgcg ggacggcgta ctgaagggcg atgttaatat
     1561 ggcactgctc ttggagggag gcggccacta ccgctgcgac ttcaagacca cctacaaagc
     1621 caagaaggtg gtgcagcttc ccgactacca cttcgtggac caccgcatcg agatcgtgag
     1681 ccacgacaag gactacaaca aagtcaagct gtacgagcac gccgaagccc acagcggact
     1741 accccgccag gccggctaat agttctagag cggccgcttc cctttagtga gggttaatgc
     1801 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1861 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1921 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1981 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atccgataag
     2041 gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2101 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2221 ttcttccctt cctttctcgc cacgttcgcg ggctttcccc gtcaagctct aaatcggggg
     2281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     2521 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     2581 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2641 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2701 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2761 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     2821 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2881 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2941 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3001 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3061 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3121 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3181 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3241 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3301 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3361 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3421 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3481 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3541 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3601 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3661 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3721 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3781 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3841 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3901 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3961 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4021 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4081 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4141 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4201 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4261 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4321 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4381 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4441 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4501 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4561 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4621 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4681 cttttgctca catggctcga cagatct
//
