LOCUS V046506 11016 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046506 VERSION V046506 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 955..5838 /label="Zfp831(NM_001099328)" /note="Zfp831(NM_001099328)" /gene="Zfp831" CDS 5875..6585 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 6611..6835 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 6881..7309 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 7323..7652 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 7719..8513 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 8689..8810 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(8859..8875) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8883..8899 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8907..8937) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8952..8973 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9261..9849) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10020..10880) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10881..10985) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggag 961 gcgtcagaac cgacccaccc taccccatcg gccacggacc agccagcctc tgtacccggc 1021 attccagggg gcctaggggg gcaggcctca cctcgcctga ccctgggtcc tgttatcctg 1081 ccaccagagc agggcctggc cccaactgtg ttcttgaagg cattgcctgt ccccttatac 1141 cacacggtac ctcctggggg cctccagcca cgtgcacccc tggtgacagg cagcctggag 1201 ggggccagcg tacctttcat tctcagcccc ttgctgcagc ctgaaggacc cagtgccacc 1261 caggtgggca agccagccac ccccgccctg actgtgaaca ttgtaggcac attgcctgta 1321 ctctcctcgg gtgtggggtc tgcactggtc agcacaggca gagtgaagaa tactggtaag 1381 tacctgtgcc cccactgtgg ccgggactgc ttgaagccca gtgtgctgga gaaacacatc 1441 cggtcccaca caggtgaaag gcccttcccg tgccctacct gtggcatcgc ctttaagacc 1501 cagagcaacc tgtataaaca caggcggaca caaacacatc tcaacaactc ccgcttgtcc 1561 ttggagtctg aggggagtgg gagcagcctt ctggaggagg gagacaggcc cggagagacc 1621 gcctcagtgg atagtcgtgg agatgaaggt gctccagaga gagccctttc cccaggtgcc 1681 ctccccgccc ttgctgctca gaacagggaa aggaaggtgg aatcggctcc ctctctcggg 1741 gccacccttt cccacaggga ggcctctgcg gatcacactc agatggtgtc acctgagttt 1801 tcactagcca gcccgcagcc taggcggaag ctgccagaac caaagccatg cttcctgcag 1861 cgacagcagg agacctgttc agagaagccc tgggacccca aggccttgga gggccagctg 1921 aggaagtgtg agagcacaga ctctggctac ctgtcgcgct cagacagcgt ggagcagccc 1981 cctgttgcct ctagcccatt gcatagtcta tcggagcaca gtgcggagtc tgagggggaa 2041 ggaggccctg gctgttcctc ttccggcaac agggctgaac gaggaacccc agggcccagc 2101 ctggagttag agaagaagaa actggaggaa cgaattgccc agctcatctc ccacaaccag 2161 gcggtggtag atgacccaca gttggaccat gtgcggcccc gcaagactgt gctgtccaag 2221 cagggcagca tcgacctgcc catgccatat acctacaagg attccttcca ctttgacatc 2281 cgggctctgg agcccagccg caggagggct gccctcagcc cggcccgctc caccttcaca 2341 cccctggaaa aggccagacc tctctttttc cactcagtcc ccacgcagct ctccactaca 2401 gtggagtgtg tccctgtcac cagaagcaac tcgctgcctt tcgtcgaggg ttctaagttg 2461 tggccagagc aacgggaccc acaggatgct gtcccaggaa tgcagaaggc cctgagcccc 2521 aggcccaccc cagcccggct gggatgccgc ccaggactga ccttggcaag catctccagt 2581 ggtcacccac gggccctggt aagacaggct gctgtggagg acctgccgtg taccccgact 2641 ggagaccctt cagccgctgc tgaggaacta gatggaaaga gagctgtggc aaaggagggt 2701 gtggtgtaca agggcagagc cagtaaaaag tgtagccaga ggaagcaaag gatgttctcc 2761 caggagaaat ggcaagtgta tggaaatgag actttcaaga gaatatacca gaaaatgaag 2821 accaaccacc agggaggaca gaaacacagg caggtgaaac agggccaggg gacagaactg 2881 gactttcctc ctcctcctcc tcctcagaaa caggggggag gaggtgagga tacaggcctg 2941 tctcaagaca gtaggatccc tatccttgga gacactacag caggatgtaa gctagagtct 3001 tggggaagta gaccatccct ggaagactca ctggaggctg agtctcctaa gcaactgaag 3061 gtggtgacca gagtcaaatc atctcctacc ctcggcagca cagatagcct ctgtttgagt 3121 ggcaagagct ctttgctctc tccaagtggg aaattagatc tgggatgcca gccgcctccg 3181 gtgcctacgg cttgccaagc aggagattta gaagctcccg agactgcttc accagactca 3241 agtggtgagg ctataaaaga aaccagccct ggagcccaaa ccgtcctcag acggtccagt 3301 gatgggtctg tggctctaca gcttgcagaa gacaagttac cttcagagag gaagagactg 3361 aaggtggaag ggctggacca tcaggagcag ccagggcctc tggaagctga tacctcagga 3421 ggctctgtgc aggctgaccc cccaccgtcc gagagacagg acagtgactc tgaggagttg 3481 ctgggttgct caaataagag taccctgcat gtgaccacag ccccggagcc ctcagatgtc 3541 tcctcagccg cttctcctgt tgccctgcaa tggccaggga taggggacaa ggattcccca 3601 ttgccttctg tacccaggac tcctgggctt cactccctct tgcctgctca gccccaggat 3661 cctggtgtcc ctaatgctcg gtcaggtggc ttctttcctc ccaagtacct cctcaggtta 3721 cctcagggag agaacccctc accgatagtt gctgcagggc ctgaaaaagg ctcagactct 3781 ctgtgtacaa gtacacagcc tgtagaacag gcccttctgg ttggatcaga actcggaatg 3841 ctccggccac ctggtgcata ctcaggccca ggtgaggcag gtggcagccc agacgaccct 3901 agctggtcca ggttaaggga tggaaaagaa gaggcacaga caggaagaga gaaaagagat 3961 aggaccaagg acaacatcct agtggttggg cactctcctg ttccaattac cagcaccacc 4021 caggagatag cctccgttct gtctactcac acctgtgaca ttcgggtggc ccaggatgta 4081 gtgagtaaga cccatgccat ccattgcctc tgcacagaca gcccaccagc aagagctagg 4141 acatatggga atctcttaaa cccttggaca tcatcccaga agctggggat accgcccaag 4201 agtgccctag aaggtcctcc ttcagaaacc aaggaagggc tgcctgcttt cttttcctct 4261 caagcacact atttccttac aacttttaca aaaccccagg atatttcctt gaaccagcag 4321 aaaccggcct tgtcacacgt cttaggaacc cccaggagct gtgaggcccc aagctccttc 4381 ccctcactta agactgaacc ccagctcaca tggtgttgct taagtcgaag cctgcccctg 4441 cctgcagagc agcggaggat agctcctgga cacttggctt catccttgcc taatagaaac 4501 ctccagggcg acagtaacaa aacactaccc aagagcaatg gaggaaggac ccagacaagc 4561 caaggaggag aagtgtctac acaggcgcct gagcttccct atccatcagt cccagagctg 4621 agggctgagg accaagtatc caggccacgg tggaagaaag aacaactatg gcgtagaaag 4681 gtgaagacat ctcgtgaaaa caccaaacag aagaaactga ggcttcattc taaaaggtgt 4741 gaagggagtt tctggcagag agccaaacgc ctgtgcaagc caccctggtt gccaagaaga 4801 agctgtctcc cccaccgact tgaggggtta gagccacggg ggacccttgg gaggagttcg 4861 gcagaagtgg caggtcgaaa tgtacaaggg gagccatctt gtgccagcaa agacttgtct 4921 gtgtctcatg ggaacaaaga aaaggaaggt gactgtagtc agacttcgag acccatgtct 4981 ccgggcatga gctcaaaaac catgttggaa atggacaaat cagctctgaa ggatatttct 5041 ccaaccactg gtgagcataa tgactgttct caggagaagg agcatgctgg tgggtccgga 5101 ctatctccac aatccaacac gttcttggca gtgtttcagg aagatgtgcc tcagggcaaa 5161 ggtcttgatg ttggacctcc agaaactcag ctgccgcctt cccaggatca agtctctatc 5221 actcccaact tgtgcttctc cccagatgtc ccagagctgc cttcttcctt taaacccaaa 5281 ggaacatttc cccaccatga cgttgctacc tctgtggctg ccatctgtgt ttctgcgggg 5341 ataagaacag gacgcaaaac cctgcgggct cacagtgcag aatcctgtgc agaggagact 5401 ctggcacgaa actccccaga tagaagaggc acaccggaca gcattccacg ggtgctcctg 5461 ccagggaggc cttcacctgg taaacaatca ccaggacaaa ctctagaaat accaagttca 5521 ggaccaagtt cagtcagttc actccaggag gaagcaagag cccaggcaga ttttccttgg 5581 tggggccagt acggatgtgg ggacctgacc gtccagggtg ctgcttcagg aagtgagagt 5641 aggacttgtc aagctgaagg attaattacc tccaagactg tcacagctcc ttctgaccca 5701 ggacaaccct cacaagtccc cgaggaggcc cctttgagat ccatccgaaa gaggagtctg 5761 gaaggaatga ggaagcagac acgggtggag ctcagcgata ccagcagtga cgatgaagac 5821 agactggtca ttgagatcct cgagtctaga gggcccttcg ggggtggagg ctctatggtg 5881 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 5941 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 6001 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 6061 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 6121 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 6181 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 6241 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 6301 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 6361 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 6421 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 6481 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 6541 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagttta aacccgctga 6601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 6661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 6721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 6781 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 6841 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 6901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 7021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 7081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 7141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 7201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 7261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 7321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 7381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 7441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 7501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 7561 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 7621 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 7681 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 7741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 7801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 7861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 7921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 7981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 8041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 8101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 8161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 8221 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 8281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 8341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 8401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 8461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 8521 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 8581 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 8641 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 8701 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 8761 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 8821 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 8881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 8941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 9001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 9061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 9121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 9181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 9241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 9301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 9481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 9721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 9841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 9901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 9961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 10021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 10081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 10141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 10201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 10261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 10321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 10381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 10441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 10501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 10561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 10621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 10681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 10741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 10801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 10861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 10921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 10981 ccgcgcacat ttccccgaaa agtgccacct gacgtc //