LOCUS V028140 9668 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028140 VERSION V028140 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 935..1588 /codon_start=1 /gene="" /product="glutathione S-transferase from Schistosoma japonicum" /transl_table=1 /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" /label="GST" CDS 1619..5188 /label="TJP2(NM_004817)" /note="TJP2(NM_004817)" /gene="TJP2" polyA_signal 5235..5459 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 5505..5933 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5947..6276 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6343..7137 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7311..7432 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7481..7497) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7505..7521 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7529..7559) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7574..7595 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7883..8468) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8639..9499) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9500..9604) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attctgcaga tatcatgtcc cctatactag gttattggaa 961 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga 1021 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg 1081 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat 1141 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg 1201 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat 1261 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat 1321 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac 1381 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg 1441 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat 1501 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac 1561 gtttggtggt ggcgaccatc ctccaaaagg tggaggcgga tcaggaggcg gtggatctat 1621 gccggtgcga ggagaccgcg ggtttccacc ccggcgggag ctgtcaggtt ggctccgcgc 1681 cccaggcatg gaagagctga tatgggaaca gtacactgtg accctacaaa aggattccaa 1741 aagaggattt ggaattgcag tgtccggagg cagagacaac ccccactttg aaaatggaga 1801 aacgtcaatt gtcatttctg atgtgctccc gggtgggcct gctgatgggc tgctccaaga 1861 aaatgacaga gtggtcatgg tcaatggcac ccccatggag gatgtgcttc attcgtttgc 1921 agttcagcag ctcagaaaaa gtgggaaggt cgctgctatt gtggtcaaga ggccccggaa 1981 ggtccaggtg gccgcacttc aggccagccc tcccctggat caggatgacc gggcttttga 2041 ggtgatggac gagtttgatg gcagaagttt ccggagtggc tacagcgaga ggagccggct 2101 gaacagccat ggggggcgca gccgcagctg ggaggacagc ccggaaaggg ggcgtcccca 2161 tgagcgggcc cggagccggg agcgggacct cagccgggac cggagccgtg gccggagcct 2221 ggagcggggc ctggaccaag accatgcgcg cacccgagac cgcagccgtg gccggagcct 2281 ggagcggggc ctggaccacg actttgggcc atcccgggac cgggaccgtg accgcagccg 2341 cggccggagc attgaccagg actacgagcg agcctatcac cgggcctacg acccagacta 2401 cgagcgggcc tacagcccgg agtacaggcg cggggcccgc cacgatgccc gctctcgggg 2461 accccgaagc cgcagccgcg agcacccgca ctcacggagc cccagccccg agcctagggg 2521 gcggccgggg cccatcgggg tcctcctgat gaaaagcaga gcgaacgaag agtatggtct 2581 ccggcttggg agtcagatct tcgtaaagga aatgacccga acgggtctgg caactaaaga 2641 tggcaacctt cacgaaggag acataattct caagatcaat gggactgtaa ctgagaacat 2701 gtctttaacg gatgctcgaa aattgataga aaagtcaaga ggaaaactac agctagtggt 2761 gttgagagac agccagcaga ccctcatcaa catcccgtca ttaaatgaca gtgactcaga 2821 aatagaagat atttcagaaa tagagtcaaa ccgatcattt tctccagagg agagacgtca 2881 tcagtattct gattatgatt atcattcctc aagtgagaag ctgaaggaaa ggccaagttc 2941 cagagaggac acgccgagca gattgtccag gatgggtgcg acacccactc cctttaagtc 3001 cacaggggat attgcaggca cagttgtccc agagaccaac aaggaaccca gataccaaga 3061 ggacccccca gctcctcaac caaaagcagc cccgagaact tttcttcgtc ctagtcctga 3121 agatgaagca atatatggcc ctaataccaa aatggtaagg ttcaagaagg gagacagcgt 3181 gggcctccgg ttggctggtg gcaatgatgt cgggatattt gttgctggca ttcaagaagg 3241 gacctcggcg gagcaggagg gccttcaaga aggagaccag attctgaagg tgaacacaca 3301 ggatttcaga ggattagtgc gggaggatgc cgttctctac ctgttagaaa tccctaaagg 3361 tgaaatggtg accattttag ctcagagccg agccgatgtg tatagagaca tcctggcttg 3421 tggcagaggg gattcgtttt ttataagaag ccactttgaa tgtgagaagg aaactccaca 3481 gagcctggcc ttcaccagag gggaggtctt ccgagtggta gacacactgt atgacggcaa 3541 gctgggcaac tggctggctg tgaggattgg gaacgagttg gagaaaggct taatccccaa 3601 caagagcaga gctgaacaaa tggccagtgt tcaaaatgcc cagagagaca acgctgggga 3661 ccgggcagat ttctggagaa tgcgtggcca gaggtctggg gtgaagaaga acctgaggaa 3721 aagtcgggaa gacctcacag ctgttgtgtc tgtcagcacc aagttcccag cttatgagag 3781 ggttttgctg cgagaagctg gtttcaagag acctgtggtc ttattcggcc ccatagctga 3841 tatagcaatg gaaaaattgg ctaatgagtt acctgactgg tttcaaactg ctaaaacgga 3901 accaaaagat gcaggatctg agaaatccac tggagtggtc cggttaaata ccgtgaggca 3961 aattattgaa caggataagc atgcactact ggatgtgact ccgaaagctg tggacctgtt 4021 gaattacacc cagtggttcc caattgtgat ttttttcaac ccagactcca gacaaggtgt 4081 caaaaccatg agacaaaggt taaatccaac gtccaacaaa agttctcgaa agttatttga 4141 tcaagccaac aagcttaaaa aaacgtgtgc acaccttttt acagctacaa tcaacctaaa 4201 ttcagccaat gatagctggt ttggcagctt aaaggacact attcagcatc agcaaggaga 4261 agcggtttgg gtctctgaag gaaagatgga agggatggat gatgaccccg aagaccgcat 4321 gtcctactta accgccatgg gcgcggacta tctgagttgc gacagccgcc tcatcagtga 4381 ctttgaagac acggacggtg aaggaggcgc ctacactgac aatgagctgg atgagccagc 4441 cgaggagccg ctggtgtcgt ccatcacccg ctcctcggag ccggtgcagc acgaggagag 4501 cataaggaaa cccagcccag agccacgagc tcagatgagg agggctgcta gcagcgatca 4561 acttagggac aatagcccgc ccccagcatt caagccagag ccgcccaagg ccaaaaccca 4621 gaacaaagaa gaatcctatg acttctccaa atcctatgaa tataagtcaa acccctctgc 4681 cgttgctggt aatgaaactc ctggggcatc taccaaaggt tatcctcctc ctgttgcagc 4741 aaaacctacc tttgggcggt ctatactgaa gccctccact cccatccctc ctcaagaggg 4801 tgaggaggtg ggagagagca gtgaggagca agataatgct cccaaatcag tcctgggcaa 4861 agtcaaaata tttgagaaga tggatcacaa ggccaggtta cagagaatgc aggagctcca 4921 ggaagcacag aatgcaagga tcgaaattgc ccagaagcat cctgatatct atgcagttcc 4981 aatcaaaacg cacaagccag accctggcac gccccagcac acgagttcca gaccccctga 5041 gccacagaaa gctccttcca gaccttatca ggataccaga ggaagttatg gcagtgatgc 5101 cgaggaggag gagtaccgcc agcagctgtc agaacactcc aagcgcggtt actatggcca 5161 gtctgcccga taccgggaca cagaattata gctcgagtct agagggcccg tttaaacccg 5221 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 5281 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 5341 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 5401 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 5461 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 5521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 5581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 5641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 5701 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 5761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 5821 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 5881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 5941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 6001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 6061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6181 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 6241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 6301 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 6361 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 6421 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 6481 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 6541 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 6601 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 6661 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 6721 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 6781 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 6841 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 6901 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 6961 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 7021 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 7081 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 7141 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 7201 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 7261 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 7321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 7381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 7441 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8401 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8461 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8521 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8581 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8641 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8701 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8761 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8821 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8881 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8941 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9001 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9061 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9121 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9181 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9241 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9301 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9361 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9421 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9481 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9541 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9601 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 9661 cctatggt //