LOCUS V041065 8176 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041065 VERSION V041065 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..3666 /label="SLFN11(NM_001104587)" /note="SLFN11(NM_001104587)" /gene="SLFN11" CDS 3673..3738 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3776..4000 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4046..4474 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4488..4817 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4884..5678 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5852..5973 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6022..6038) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6046..6062 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6070..6100) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6115..6136 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6424..7009) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7180..8040) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8041..8145) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatggagg caaatcagtg ccccctggtt gtggaaccat cttacccaga cctggtcatc 1021 aatgtaggag aagtgactct tggagaagaa aacagaaaaa agctgcagaa aattcagaga 1081 gaccaagaga aggagagagt tatgcgggct gcatgtgctt tattaaactc aggaggagga 1141 gtgattcgaa tggccaagaa ggttgagcat cccgtggaga tgggactgga tttagaacag 1201 tctttgagag agcttattca gtcttcagat ctgcaggctt tctttgagac caagcaacaa 1261 ggaaggtgtt tttacatttt tgttaaatct tggagcagtg gccctttccc tgaagatcgc 1321 tctgtcaagc cccgcctttg cagcctcagt tcttcattat accgtagatc tgagacctct 1381 gtgcgttcca tggactcaag agaggcattc tgtttcctga agaccaaaag gaagccaaaa 1441 atcttggaag aaggaccttt tcacaaaatt cacaagggtg tataccaaga gctccctaac 1501 tcggatcctg ctgacccaaa ctcggatcct gctgacctaa ttttccaaaa agactatctt 1561 gaatatggtg aaatcctgcc ttttcctgag tctcagttag tagagtttaa acagttctct 1621 acaaaacact tccaagaata tgtaaaaagg acaattccag aatacgtccc tgcatttgca 1681 aacactggag gaggctatct ttttattgga gtggatgata agagtaggga agtcctggga 1741 tgtgcaaaag aaaatgttga ccctgactct ttgagaagga aaatagaaca agccatatac 1801 aaactacctt gtgttcattt ttgccaaccc caacgcccga taaccttcac actcaaaatt 1861 gtgaatgtgt taaaaagggg agagctctat ggctatgctt gcatgatcag agtaaatccc 1921 ttctgctgtg cagtgttctc agaagctccc aattcatgga tagtggagga caagtacgtc 1981 tgcagcctga caaccgagaa atgggtaggc atgatgacag acacagatcc agatcttcta 2041 cagttgtctg aagattttga atgtcagctg agtctatcta gtgggcctcc ccttagcaga 2101 ccagtgtact ccaagaaagg cctggaacat aaaaaggaac tccagcaact tttattttca 2161 gtcccaccag gatatttgcg atatactcca gagtcactct ggagggacct gatctcagag 2221 cacagaggac tagaggagtt aataaataag caaatgcaac ctttctttcg gggaattttg 2281 atcttctcta gaagttgggc tgtggacctg aacttgcagg agaagccagg agtcatctgt 2341 gatgctctgc tgatagcaca gaacagcacc cccattctct acaccattct cagggagcaa 2401 gatgcagagg gccaggacta ctgcactcgc accgccttta ctttgaagca gaagctagtg 2461 aacatggggg gctacaccgg gaaggtgtgt gtcagggcca aggtcctctg cctgagtcct 2521 gagagcagcg cagaggcctt ggaggctgca gtgtctccga tggattaccc tgcgtcctat 2581 agccttgcag gcacccagca catggaagcc ctgctgcagt ccctcgtgat tgtcttactc 2641 ggcttcaggt ctctcttgag tgaccagctc ggctgtgagg ttttaaatct gctcacagcc 2701 cagcagtatg agatattctc cagaagcctc cgcaagaaca gagagttgtt tgtccacggc 2761 ttacctggct cagggaagac catcatggcc atgaagatca tggagaagat caggaatgtg 2821 tttcactgtg aggcacacag aattctctac gtttgtgaaa accagcctct gaggaacttt 2881 atcagtgata gaaatatctg ccgagcagag acccggaaaa ctttcctaag agaaaacttt 2941 gaacacattc aacacatcgt cattgacgaa gctcagaatt tccgtactga agatggggac 3001 tggtatggga aggcaaaaag catcactcgg agagcaaagg gtggcccagg aattctctgg 3061 atctttctgg attactttca gaccagccac ttggattgca gtggcctccc tcctctctca 3121 gaccaatatc caagagaaga gctcaccaga atagttcgca atgcagatcc aatagccaag 3181 tacttacaaa aagaaatgca agtaattaga agtaatcctt catttaacat ccccactggg 3241 tgcctcgagg tatttcctga agccgaatgg tcccagggtg ttcagggaac cttacgaatt 3301 aagaaatact tgactgtgga gcaaataatg acctgtgtgg cagacacgtg caggcgcttc 3361 tttgataggg gctattctcc aaaggatgtt gctgtgcttg tcagcaccgc aaaagaagtg 3421 gagcactata agtatgagct cttgaaagca atgaggaaga aaagggtggt gcagctcagt 3481 gatgcatgtg atatgttggg tgatcacatt gtgttggaca gtgttcggcg attctcaggc 3541 ctggaaagga gcatagtgtt tgggatccat ccaaggacag ctgacccagc tatcttaccc 3601 aatgttctga tctgtctggc ttccagggca aaacaacacc tgtatatttt tccgtggggt 3661 ggccatctcg aggactacaa agaccatgac ggtgattata aagatcatga catcgactac 3721 aaggatgacg atgacaagta gtgagggccc gtttaaaccc gctgatcagc ctcgactgtg 3781 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 3841 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 3901 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 3961 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 4021 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 4081 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 4141 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 4201 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 4261 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 4321 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 4381 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 4441 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 4501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 4561 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 4621 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 4681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 4741 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 4801 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 4861 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 4921 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 4981 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 5041 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 5101 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 5161 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 5221 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 5281 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 5341 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 5401 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 5461 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 5521 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 5581 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 5641 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 5701 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 5761 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 5821 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 5881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 5941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 6001 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 6061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8161 agtgccacct gacgtc //