LOCUS V026803 7715 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026803 VERSION V026803 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3205 /label="SENP5(NM_152699)" /note="SENP5(NM_152699)" /gene="SENP5" CDS 3212..3277 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3315..3539 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3585..4013 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4027..4356 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4423..5217 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5391..5512 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5561..5577) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5585..5601 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5609..5639) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5654..5675 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5963..6548) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6719..7579) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7580..7684) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaaaaaac agaggaaaat 961 tctatggagg aaaggaatcc acttagcctt ttctgagaaa tggaatactg ggtttggagg 1021 ctttaagaag ttttattttc accaacactt gtgcattctg aaagctaagc tgggaaggcc 1081 agttacttgg aatagacagt tgagacattt ccagggtaga aagaaagctc ttcaaatcca 1141 gaaaacgtgg atcaaggatg aacccctttg tgctaagacc aagttcaatg tggctactca 1201 aaatgttagt actttgtcct ctaaagtgaa aagaaaggac gctaaacact tcatttcctc 1261 ctcaaagact ctcctgagac tccaagcaga gaagctgttg tcatcagcaa agaattctga 1321 ccatgaatac tgcagagaga aaaatctctt gaaggcagtt actgactttc catcaaatag 1381 tgctttaggt caggccaatg gtcacagacc taggacagac ccacaacctt ctgactttcc 1441 catgaagttc aatggggaga gccaaagtcc aggtgagagt ggcacgattg tggtcacctt 1501 gaacaaccat aagagaaagg gcttttgtta cggctgctgc caagggccgg agcaccacag 1561 gaatggggga cccttgattc caaaaaagtt ccaacttaac caacatagaa ggataaaatt 1621 atctcctctt atgatgtatg agaaattatc catgattaga tttcggtaca ggattctcag 1681 atcccagcac ttcagaacca aaagcaaggt ttgcaagcta agaaaagccc agcgaagctg 1741 ggtacagaaa gtcactgggg accatcaaga gacccgtagg gagaacggtg agggtggcag 1801 ttgcagccca tttccttccc cagaacctaa agacccttct tgtcggcatc agccgtactt 1861 tccagatatg gacagcagtg ctgtggtgaa ggggacgaac tctcatgtgc ctgattgcca 1921 cactaaagga agctctttct tgggcaagga gcttagttta gacgaagcat tccctgacca 1981 acagaatggc agtgccacaa acgcctggga ccagtcatcc tgttcttctc ctaagtggga 2041 gtgtacagag ctgattcatg acatcccctt accagaacat cgttctaata ccatgttcat 2101 ttcagaaact gaaagagaaa ttatgactct gggtcaggaa aatcagacaa gttctgtcag 2161 tgatgacaga gtaaaactgt cagtgtctgg agcagataca tctgtgagta gcgtagatgg 2221 gcctgtgtcc caaaaggctg ttcaaaatga gaactcatac cagatggagg aggatggatc 2281 tctcaagcag agcattctta gttctgagtt gctggaccac ccttactgta aaagtccact 2341 ggaggctccc ttggtgtgca gtggactcaa actagaaaat caagtaggag gtggaaagaa 2401 cagtcagaaa gcctctccag tggatgatga acagctgtca gtctgtcttt ctggattcct 2461 agatgaggtt atgaagaagt atggcagttt ggttccactc agtgaaaaag aagtccttgg 2521 aagattaaaa gatgtcttta atgaagactt ttctaataga aaaccattta tcaataggga 2581 aataacaaac tatcgggcca gacatcaaaa atgtaacttc cgtatcttct ataataaaca 2641 catgctggat atggacgacc tggcgactct ggatggtcag aactggctga atgaccaggt 2701 cattaatatg tatggtgagc tgataatgga tgcagtccca gacaaagttc acttcttcaa 2761 cagctttttt catagacagc tggtaaccaa aggatataat ggagtaaaaa gatggactaa 2821 aaaggtggat ttgtttaaaa agagtcttct gttgattcct attcacctgg aagtccactg 2881 gtctctcatt actgtgacac tctctaatcg aattatttca ttttatgatt cccaaggcat 2941 tcattttaag ttttgtgtag agaatataag aaagtatttg ctgactgaag ccagagaaaa 3001 aaatagacct gaatttcttc agggttggca gactgctgtt acgaagtgta ttccacaaca 3061 gaaaaacgac agtgactgtg gagtctttgt gctccagtac tgcaagtgcc tcgccttaga 3121 gcagcctttc cagttttcac aagaagacat gccccgagtg cggaagagga tttacaagga 3181 gctatgtgag tgccggctca tggacctcga ggactacaaa gaccatgacg gtgattataa 3241 agatcatgac atcgactaca aggatgacga tgacaagtag tgagggcccg tttaaacccg 3301 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 3361 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 3421 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 3481 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 3541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 3601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 3661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 3721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 3781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 3841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 3901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 3961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 4021 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4081 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 4141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 4201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 4261 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 4321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 4381 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 4441 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 4501 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 4561 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 4621 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 4681 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 4741 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 4801 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 4861 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 4921 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 4981 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 5041 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 5101 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 5161 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 5221 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 5281 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 5341 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 5401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5521 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 5581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 6001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 6061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 6121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 6181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 6421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6481 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 6961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7681 cgcgcacatt tccccgaaaa gtgccacctg acgtc //