LOCUS V037655 6139 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037655 VERSION V037655 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 932..1654 /label="Pacrg(NM_027032)" /note="Pacrg(NM_027032)" /gene="Pacrg" CDS 1676..1705 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" polyA_signal 1734..1958 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2004..2432 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2446..2775 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2842..3636 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3812..3933 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3982..3998) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4006..4022 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4030..4060) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4075..4096 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4384..4972) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5143..6003) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6004..6108) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccgccac catgccgaag aggactaaac tgctgccaca 961 acagacgttc caggtgcacc agcctcgttc cctggtttct gagggcttca cagtcaaagc 1021 catgatgaaa aactcagtcg tgcggggtcc tccagttgca ggggcattta aagaaaggcc 1081 ggccaaaccc accactttcc gaaaatgtta tgagcgagga gacttcccaa tcgcccttga 1141 gcatgactcg aaaggaaaca aaattgcctg gaaggttgag attgagaagt tggactacca 1201 tcattacctg cctctgtttt ttgatgggct ttctgaaatg acgtttccct atgagttttt 1261 tgctcggcga ggaatccacg acatgctgga acacggaggg aacaagattc tacctgtcat 1321 tcctcagctc attatcccaa taaaaaatgc cttgaacctc cgaaacagac agatcatctg 1381 tgtgacgctc aaggttctcc agcatctggt tgtgtcttcg gagatggtgg gcgaagcttt 1441 gttgccctac taccgtcaga tcctgccaat cctgaacatc tttaagaaca tgaatgtgaa 1501 ctccggggat ggcatcgact acagccagca gaagagggag aacattggcg acctgatcca 1561 ggagacccta gaagccttcg agcgctacgg aggggaggac gccttcatca acatcaaata 1621 catggtgcct acctatgagt cgtgcttgct gaacctcgag tctagagggc ccttcgaaca 1681 aaaactcatc tcagaagagg atctgtgagt ttaaacccgc tgatcagcct cgactgtgcc 1741 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 1801 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 1861 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 1921 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 1981 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2041 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2101 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2161 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2221 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 2281 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 2341 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 2401 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 2461 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2521 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2581 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2641 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 2701 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 2761 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 2821 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 2881 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 2941 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3001 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 3061 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3121 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3181 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3241 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3301 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3361 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3421 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3481 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3541 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3601 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat 3661 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 3721 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 3781 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 3841 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3901 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 3961 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4021 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4081 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4141 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4201 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4261 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4801 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 4861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5041 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5101 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 5161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5221 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5281 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 5581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 5641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 5761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 6001 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 6061 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6121 aaaagtgcca cctgacgtc //