LOCUS V035129 6388 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035129 VERSION V035129 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1827 /label="PHB2(NM_001144831)" /note="PHB2(NM_001144831)" /gene="PHB2" CDS 1834..1860 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1864..1890 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1894..1920 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1955..2179 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2225..2653 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2667..2996 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3063..3857 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4031..4152 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4201..4217) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4225..4241 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4249..4279) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4294..4315 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4603..5188) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5359..6219) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6220..6324) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atggcccaga acttgaagga cttggcggga 961 cggctgcccg ccgggccccg gggcatgggc acggccctga agctgttgct gggggccggc 1021 gccgtggcct acggtgtgcg cgaatctgtg ttcaccgtgg aaggcgggca cagagccatc 1081 ttcttcaatc ggatcggtgg agtgcagcag gacactatcc tggccgaggg ccttcacttc 1141 aggatccctt ggttccagta ccccattatc tatgacattc gggccagacc tcgaaaaatc 1201 tcctccccta caggctccaa agacctacag atggtgaata tctccctgcg agtgttgtct 1261 cgacccaatg ctcaggagct tcctagcatg taccagcgcc tagggctgga ctacgaggaa 1321 cgagtgttgc cgtccattgt caacgaggtg ctcaagagtg tggtggccaa gttcaatgcc 1381 tcacagctga tcacccagcg ggcccaggta tccctgttga tccgccggga gctgacagag 1441 agggccaagg acttcagcct catcctggat gatgtggcca tcacagagct gagctttagc 1501 cgagagtaca cagctgctgt agaagccaaa caagtggccc agcaggaggc ccagcgggcc 1561 caattcttgg tagaaaaagc aaagcaggaa cagcggcaga aaattgtgca ggccgagggt 1621 gaggccgagg ctgccaagat gcttggagaa gcactgagca agaaccctgg ctacatcaaa 1681 cttcgcaaga ttcgagcagc ccagaatatc tccaagacga tcgccacatc acagaatcgt 1741 atctatctca cagctgacaa ccttgtgctg aacctacagg atgaaagttt caccagggga 1801 agtgacagcc tcatcaaggg taagaaactc gagtacccat acgacgtacc tgattatgca 1861 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca 1921 tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 1981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 2041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 2101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 2161 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 2221 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2281 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2341 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 2401 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 2461 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 2521 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 2581 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 2641 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 2761 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2821 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2881 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 2941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 3001 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 3061 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 3121 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 3181 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 3241 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 3301 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3361 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3421 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 3481 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 3541 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 3601 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 3661 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 3721 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 3781 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 3841 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 3901 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 3961 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 4021 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 4081 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 4141 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 4201 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 4261 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 4321 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 4381 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 4441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 4501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 4621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 4681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 4741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 4801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 4861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 4921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 4981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 5041 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 5101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca 5161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 5461 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 5521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 5581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 5641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 5701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 5761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 5821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 6001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 6061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 6121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 6181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 6241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 6301 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 6361 atcgggagat ctcccgatcc cctatggt //