LOCUS V018369 6004 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018369 VERSION V018369 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..1530 /label="PARK7(NM_007262)" /note="PARK7(NM_007262)" /gene="PARK7" CDS 1531..1557 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1604..1828 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1874..2302 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2316..2645 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2712..3506 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3680..3801 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3850..3866) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3874..3890 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3898..3928) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3943..3964 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4252..4837) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5008..5868) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5869..5973) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatggctt ccaaaagagc tctggtcatc ctggctaaag gagcagagga aatggagacg 1021 gtcatccctg tagatgtcat gaggcgagct gggattaagg tcaccgttgc aggcctggct 1081 ggaaaagacc cagtacagtg tagccgtgat gtggtcattt gtcctgatgc cagccttgaa 1141 gatgcaaaaa aagagggacc atatgatgtg gtggttctac caggaggtaa tctgggcgca 1201 cagaatttat ctgagtctgc tgctgtgaag gagatactga aggagcagga aaaccggaag 1261 ggcctgatag ccgccatctg tgcaggtcct actgctctgt tggctcatga aataggtttt 1321 ggaagtaaag ttacaacaca ccctcttgct aaagacaaaa tgatgaatgg aggtcattac 1381 acctactctg agaatcgtgt ggaaaaagac ggcctgattc ttacaagccg ggggcctggg 1441 accagcttcg agtttgcgct tgcaattgtt gaagccctga atggcaagga ggtggcggct 1501 caagtgaagg ctccacttgt tcttaaagac tatccttatg acgtgcctga ctatgcctga 1561 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 1621 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 1681 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 1741 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 1801 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 1861 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 1921 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 1981 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 2041 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 2101 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 2161 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 2221 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 2281 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 2341 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2401 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2461 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 2521 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 2581 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 2641 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 2701 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 2761 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 2821 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 2881 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 2941 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 3001 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 3061 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 3121 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 3181 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 3241 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 3301 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 3361 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 3421 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 3481 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 3541 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 3601 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 3661 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 3721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3781 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 3841 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 3901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 3961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 4021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 4081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 4141 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 4201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 4261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 4321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 4381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 4441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 4501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 4561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 4621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 4681 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 4741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 4801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 5041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 5101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 5161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 5221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 5281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 5341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 5401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 5461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 5521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 6001 cgtc //