LOCUS       V044947                 7948 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044947
VERSION     V044947
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3387
                     /label="NUP93(NM_014669)"
                     /note="NUP93(NM_014669)"
                     /gene="NUP93"
     CDS             3394..3420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3424..3450
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3454..3480
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3515..3739
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3785..4213
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4227..4556
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4623..5417
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5591..5712
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5761..5777)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5785..5801
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5809..5839)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5854..5875
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6163..6748)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6919..7779)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7780..7884)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggatactg aggggtttgg tgagctcctt
      961 cagcaagctg aacagcttgc tgctgagact gagggcatct cagagcttcc ccatgtggaa
     1021 cggaacttac aggagatcca gcaggcggga gagcgcctgc gttcccgtac cctaacacgc
     1081 acgtcccagg agacggcaga tgtcaaggcg tcagttctcc tcgggtctcg gggacttgac
     1141 atatcccaca tctcccagcg attggagagt ctgagtgcag ccaccacctt tgagcctctt
     1201 gagcctgtga aggacactga cattcagggc ttcctgaaga atgagaagga caatgccctg
     1261 ctgtctgcca tcgaagagtc ccggaagagg accttcggca tggctgagga gtaccatcgg
     1321 gagtcaatgt tggttgagtg ggagcaagtg aaacagcgaa ttctgcacac actgctggca
     1381 tcaggagaag acgcccttga ctttactcaa gaaagcgagc caagctacat cagtgatgtg
     1441 ggaccccctg gtcgaagctc tctggataac atcgagatgg cctatgcgcg gcaaatttat
     1501 atctataatg agaaaattgt aaatggacac ctgcagccta acctggtgga cctttgtgct
     1561 tccgtcgcag agctcgatga taagagcatt tccgacatgt ggaccatggt aaaacaaatg
     1621 acagacgtgt tgttgacacc ggcaacggat gccctgaaga accgcagcag cgtggaagtg
     1681 cgcatggagt ttgtcaggca ggccttggcg taccttgagc agagttataa gaattacacc
     1741 cttgtgactg tctttggaaa tttgcatcag gcccagctgg gcggggtgcc tgggacttac
     1801 caattggttc gaagtttcct gaacattaaa ctgccagctc ccttgcctgg actacaggat
     1861 ggagaggtgg aaggccatcc tgtgtgggcg ctaatttact actgcatgcg ctgtggagac
     1921 ctgcttgccg cttcacaggt agttaatcga gcccagcacc agctgggaga gtttaaaacc
     1981 tggttccagg agtacatgaa cagcaaggac agaagattgt ccccagctac ggaaaacaag
     2041 ctccggctgc attaccgtag ggccctcagg aacaatacag atccctacaa gcgggccgtg
     2101 tactgtatca ttggcagatg tgacgtcacc gacaaccaga gtgaagtggc ggacaaaact
     2161 gaggattacc tgtggctgaa gttgaaccaa gtgtgttttg acgacgatgg caccagctcc
     2221 ccacaagaca ggctcactct ctcacagttc cagaagcagt tgttggaaga ctatggcgag
     2281 tcccacttta cggtgaacca gcaacccttc ctctacttcc aagtcctgtt cctgacagcg
     2341 cagtttgaag cagcagttgc ctttcttttc cgcatggagc ggctgcgctg ccatgctgtc
     2401 catgtagcac tggtgctgtt tgagctgaag ctgcttttaa agtcctctgg acagagtgct
     2461 cagctcctca gccacgagcc tggtgaccct ccttgcttgc ggcggctgaa cttcgtgcgg
     2521 ctcctcatgc tgtacacccg gaagtttgag tccacggacc caagggaggc cctccagtac
     2581 ttctatttcc tcagggatga gaaagatagt caaggagaaa acatgtttct gcgctgtgtg
     2641 agtgagcttg tgattgaaag ccgagagttc gatatgattc ttgggaaact agagaatgac
     2701 ggaagtagaa agcctggagt catagataag tttactagtg acacaaagcc tattatcaac
     2761 aaagttgctt ctgtggcaga aaataaagga ctgtttgaag aggcagcaaa gctgtatgac
     2821 cttgccaaga atgctgacaa ggtactggag ctgatgaaca aactgctgag ccctgtcgtc
     2881 ccccagatca gtgccccgca atccaacaag gagaggctga agaacatggc actctccatt
     2941 gccgaacggt atagggctca aggaataagc gcaaataaat ttgtggactc cacgttctat
     3001 cttcttttgg acttgatcac cttttttgac gagtatcata gtggtcatat tgatagagct
     3061 tttgatatca ttgagcgctt gaagctggtg cccctgaatc aggaaagtgt ggaagagaga
     3121 gtggctgcct tcagaaattt cagtgatgaa atcaggcaca acctctcaga agtgcttctt
     3181 gccaccatga acatcttgtt cacacagttt aagaggctca aggggacaag tccatcctcg
     3241 tcatccaggc cccagcgagt catcgaggac cgcgactctc aactccgaag tcaagcccgc
     3301 actctgatta cctttgctgg aatgatacca taccgaacgt ctggggacac caatgcgagg
     3361 ctggtgcaga tggaggtcct catgaatctc gagtacccat acgacgtacc tgattatgca
     3421 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     3481 tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3721 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3781 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4021 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4081 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4141 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4201 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4561 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4621 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4681 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4741 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4801 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4861 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4921 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4981 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5041 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5101 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5161 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5221 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5281 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5341 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5401 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5461 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5521 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5581 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5641 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5701 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6601 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7921 atcgggagat ctcccgatcc cctatggt
//
