LOCUS V047414 7584 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047414 VERSION V047414 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 905..3025 /label="FBXW7(NM_033632)" /note="FBXW7(NM_033632)" /gene="FBXW7" CDS 3041..3082 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" polyA_signal 3184..3408 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3454..3882 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3896..4225 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4292..5086 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5260..5381 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5430..5446) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5454..5470 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5478..5508) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5523..5544 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5832..6417) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6588..7448) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7449..7553) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 caccatgaat caggaactgc tctctgtggg cagcaaaaga cgacgaactg gaggctctct 961 gagaggtaac ccttcctcaa gccaggtaga tgaagaacag atgaatcgtg tggtagagga 1021 ggaacagcaa cagcaactca gacaacaaga ggaggagcac actgcaagga atggtgaagt 1081 tgttggagta gaacctagac ctggaggcca aaatgattcc cagcaaggac agttggaaga 1141 aaacaataat agatttattt cggtagatga ggactcctca ggaaaccaag aagaacaaga 1201 ggaagatgaa gaacatgctg gtgaacaaga tgaggaggat gaggaggagg aggagatgga 1261 ccaggagagt gacgattttg atcagtctga tgatagtagc agagaagatg aacatacaca 1321 tactaacagt gtcacgaact ccagtagtat tgtggacctg cccgttcacc aactctcctc 1381 cccattctat acaaaaacaa caaaaatgaa aagaaagttg gaccatggtt ctgaggtccg 1441 ctctttttct ttgggaaaga aaccatgcaa agtctcagaa tatacaagta ccactgggct 1501 tgtaccatgt tcagcaacac caacaacttt tggggacctc agagcagcca atggccaagg 1561 gcaacaacga cgccgaatta catctgtcca gccacctaca ggcctccagg aatggctaaa 1621 aatgtttcag agctggagtg gaccagagaa attgcttgct ttagatgaac tcattgatag 1681 ttgtgaacca acacaagtaa aacatatgat gcaagtgata gaaccccagt ttcaacgaga 1741 cttcatttca ttgctcccta aagagttggc actctatgtg ctttcattcc tggaacccaa 1801 agacctgcta caagcagctc agacatgtcg ctactggaga attttggctg aagacaacct 1861 tctctggaga gagaaatgca aagaagaggg gattgatgaa ccattgcaca tcaagagaag 1921 aaaagtaata aaaccaggtt tcatacacag tccatggaaa agtgcataca tcagacagca 1981 cagaattgat actaactgga ggcgaggaga actcaaatct cctaaggtgc tgaaaggaca 2041 tgatgatcat gtgatcacat gcttacagtt ttgtggtaac cgaatagtta gtggttctga 2101 tgacaacact ttaaaagttt ggtcagcagt cacaggcaaa tgtctgagaa cattagtggg 2161 acatacaggt ggagtatggt catcacaaat gagagacaac atcatcatta gtggatctac 2221 agatcggaca ctcaaagtgt ggaatgcaga gactggagaa tgtatacaca ccttatatgg 2281 gcatacttcc actgtgcgtt gtatgcatct tcatgaaaaa agagttgtta gcggttctcg 2341 agatgccact cttagggttt gggatattga gacaggccag tgtttacatg ttttgatggg 2401 tcatgttgca gcagtccgct gtgttcaata tgatggcagg agggttgtta gtggagcata 2461 tgattttatg gtaaaggtgt gggatccaga gactgaaacc tgtctacaca cgttgcaggg 2521 gcatactaat agagtctatt cattacagtt tgatggtatc catgtggtga gtggatctct 2581 tgatacatca atccgtgttt gggatgtgga gacagggaat tgcattcaca cgttaacagg 2641 gcaccagtcg ttaacaagtg gaatggaact caaagacaat attcttgtct ctgggaatgc 2701 agattctaca gttaaaatct gggatatcaa aacaggacag tgtttacaaa cattgcaagg 2761 tcccaacaag catcagagtg ctgtgacctg tttacagttc aacaagaact ttgtaattac 2821 cagctcagat gatggaactg taaaactatg ggacttgaaa acgggtgaat ttattcgaaa 2881 cctagtcaca ttggagagtg gggggagtgg gggagttgtg tggcggatca gagcctcaaa 2941 cacaaagctg gtgtgtgcag ttgggagtcg gaatgggact gaagaaacca agctgctggt 3001 gctggacttt gatgtggaca tgaagggtgg aggcggttca ggtaagccta tccctaaccc 3061 tctcctcggt ctcgattcta cataggatcc actagtccag tgtggtggaa ttctgcagat 3121 atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct 3181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 3241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 3301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 3361 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 3421 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 3481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 3541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 3601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 3661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 3721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 3781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 3841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 3901 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3961 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 4021 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 4081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 4141 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 4201 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4441 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4801 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 5101 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 5161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 5221 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat 5281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg 5401 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 5461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 5521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 5581 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 5641 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 5701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 6001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 6061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 6121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 6181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 6301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6361 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 6421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 6481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 6541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 6601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 6661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 6721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 6781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 6841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 6901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 6961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 7021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 7081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 7141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 7201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 7261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 7321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 7381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 7441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 7501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 7561 ccccgaaaag tgccacctga cgtc //