LOCUS V038046 6847 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038046 VERSION V038046 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 917..2317 /label="DMAP1(NM_019100)" /note="DMAP1(NM_019100)" /gene="DMAP1" CDS 2318..2344 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 2447..2671 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2717..3145 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3159..3488 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3555..4349 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4523..4644 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4693..4709) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4717..4733 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4741..4771) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4786..4807 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5095..5680) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5851..6711) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6712..6816) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttatgg ctacgggcgc ggatgtacgg gacattctag aactcggggg 961 tccagaaggg gatgcagcct ctgggaccat cagcaagaag gacattatca acccggacaa 1021 gaaaaaatcc aagaagtcct ctgagacact gactttcaag aggcccgagg gcatgcaccg 1081 ggaagtctat gccttgctct actctgacaa gaaggatgca cccccactgc tacccagtga 1141 cactggccag ggataccgta cagtgaaggc caagttgggc tccaagaagg tgcggccttg 1201 gaagtggatg ccattcacca acccggcccg caaggacgga gcaatgttct tccactggcg 1261 acgtgcagcg gaggagggca aggactaccc ctttgccagg ttcaataaga ctgtgcaggt 1321 gcctgtgtac tcggagcagg agtaccagct ttatctccac gatgatgctt ggactaaggc 1381 agaaactgac cacctctttg acctcagccg ccgctttgac ctgcgttttg ttgttatcca 1441 tgaccggtat gaccaccagc agttcaagaa gcgttctgtg gaagacctga aggagcggta 1501 ctaccacatc tgtgctaagc ttgccaacgt gcgggctgtg ccaggcacag accttaagat 1561 accagtattt gatgctgggc acgaacgacg gcggaaggaa cagcttgagc gtctctacaa 1621 ccggacccca gagcaggtgg cagaggagga gtacctgcta caggagctgc gcaagattga 1681 ggcccggaag aaggagcggg agaaacgcag ccaggacctg cagaagctga tcacagcggc 1741 agacaccact gcagagcagc ggcgcacgga acgcaaggcc cccaaaaaga agctacccca 1801 gaaaaaggag gctgagaagc cggctgttcc tgagactgca ggcatcaagt ttccagactt 1861 caagtctgca ggtgtcacgc tgcggagcca acggatgaag ctgccaagct ctgtgggaca 1921 gaagaagatc aaggccctgg aacagatgct gctggagctt ggtgtggagc tgagcccgac 1981 acctacggag gagctggtgc acatgttcaa tgagctgcga agcgacctgg tgctgctcta 2041 cgagctcaag caggcctgtg ccaactgcga gtatgagctg cagatgctgc ggcaccgtca 2101 tgaggcactg gcccgggctg gtgtgctagg gggccctgcc acaccagcat caggcccagg 2161 cccggcctct gctgagccgg cagtgactga acccggactt ggtcctgacc ccaaggacac 2221 catcattgat gtggtgggcg cacccctcac gcccaattcg agaaagcgac gggagtcggc 2281 ctccagctca tcttccgtga agaaagccaa gaagccgtac ccatacgatg ttccagatta 2341 cgcttaagga tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg 2401 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 2461 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 2521 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 2581 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 2641 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 2701 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 2761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 2821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 2881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 2941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 3001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 3061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 3121 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 3181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3241 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3421 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3481 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 3541 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 3601 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 3661 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 3721 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 3781 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 3841 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 3901 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 3961 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4021 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4081 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 4141 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4201 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4261 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4321 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4381 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4441 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4501 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 4561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 4621 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 4681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 4741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 4801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 4861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 5041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 5641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 5761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 5821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 5881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 5941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6841 tgacgtc //