LOCUS       V022503                 9164 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022503
VERSION     V022503
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4684
                     /label="AUTS2(NM_015570)"
                     /note="AUTS2(NM_015570)"
                     /gene="AUTS2"
     polyA_signal    4731..4955
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5001..5429
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5443..5772
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5839..6633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6807..6928
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6977..6993)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7001..7017
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7025..7055)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7070..7091
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7379..7964)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8135..8995)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8996..9100)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gatggcccga cgcggggcca tggactccgc aaaaagcggc ggtcgcggtc
      961 gcagcgagac cgggagaggc gctcccgggg cgggctgggg gccggcgcgg ccggcggcgg
     1021 cggggctggc cggacccggg cgctctcact cgcctcgtcg tcgggctccg acaaggaaga
     1081 caatgggaag cccccgtcct ccgccccgtc ccggcccaga cccccgcgga ggaagcggag
     1141 agagtccacc tcggcagaag aggacatcat tgatggattt gccatgacca gctttgtcac
     1201 ttttgaagcg ctggagaaag atgtagcact taagcctcag gaacgtgtgg agaaacgcca
     1261 gacgcccctg accaagaaga aacgagaagc acttaccaat ggcttgtcct ttcattcaaa
     1321 gaagagcaga ctcagccacc cacaccacta cagctcagat cgagaaaatg accgcaatct
     1381 ctgccagcac cttgggaaga gaaagaaaat gccgaaggca ctcagacagc tcaagccagg
     1441 acagaacagc tgcagggaca gtgacagtga aagtgccagt ggagaatcca agggcttcca
     1501 ccggagcagc tctcgggaaa ggctcagtga tagttcagct ccttccagct tgggaacagg
     1561 ctacttctgt gacagtgaca gtgaccagga agagaaggca tcagatgcca gctctgaaaa
     1621 actcttcaac actgttattg taaacaaaga tccggagtta ggtgttggca cgctaccaga
     1681 acatgacagc caggatgcag ggccgattgt ccccaagata tcgggtctag agagaagcca
     1741 ggagaagagc caggactgtt gcaaagagcc aatctttgag cctgtggtgc ttaaagaccc
     1801 ctgccctcag gtcgcacagc caatacccca gccgcagacg gagccccaac tccgagctcc
     1861 ttctccggac cctgacttgg tgcagcgcac agaggcccca cctcaacccc cacctctgag
     1921 tacacagcca ccacagggcc ctcctgaggc ccagctccag cctgccccgc agcctcaggt
     1981 gcagaggcca cccaggccac agtcccccac ccagctgctc catcagaacc tcccacctgt
     2041 gcaggcccac ccctctgctc agagcctctc ccagccattg tcagcctaca acagcagtag
     2101 cttaagcctc aacagtttaa gcagcagcag aagcagcact ccagcgaaga ctcagcccgc
     2161 cccacctcac atctcccacc acccctctgc ctccccgttc cccctctccc tgcccaacca
     2221 cagccccctg cacagcttca cacccaccct ccagcccccc gcacactcac atcaccccaa
     2281 tatgtttgcc cctcccactg ctctgcctcc tccaccacca ctgacatcag gaagtctgca
     2341 ggtggccgga cacccggccg ggagcactta ctcagagcaa gacatcttgc gacaggaact
     2401 gaacactcgt tttttggcct ctcagagtgc tgaccgcggg gcttccctgg gccctccgcc
     2461 ctacctgcgg accgagttcc atcagcacca gcaccagcac cagcacaccc accagcacac
     2521 gcaccagcac accttcacgc cgttccccca cgccatccca cccaccgcca tcatgccgac
     2581 gccagcacct cccatgtttg acaaataccc tacaaaagtt gacccattct accggcacag
     2641 tctcttccat tcctatcctc ctgcagtgtc gggcatcccc cctatgatcc cacccactgg
     2701 cccttttggt tcactacaag gagcatttca gccgaagaca tccaacccta tcgatgtcgc
     2761 tgctcggcct gggacagtcc cacacacttt actccaaaag gacccgaggt tgacagatcc
     2821 tttcagacct atgttaagga aaccagggaa gtggtgtgct atgcatgttc acatcgcctg
     2881 gcagatttac caccaccaac agaaagtcaa gaaacagatg cagtcagacc cacataagct
     2941 ggactttgga ctgaaacctg agttcctgag ccgccctcca ggccccagtc tttttggagc
     3001 catccaccac ccccatgacc tggcacggcc ttcaactttg ttctctgccg ctggtgctgc
     3061 acacccaact gggacccctt ttgggccacc tcctcatcac agcaacttcc tcaaccctgc
     3121 tgcccaccta gagcctttta atcggccgtc tacattcaca ggcctagcag cagttggtgg
     3181 caatgccttc gggggacttg gaaatccttc cgttacaccc aactcaatgt tcggccacaa
     3241 ggatggcccc agtgtgcaga actttagcaa ccctcacgaa ccctggaacc ggctgcaccg
     3301 aacgcctccg tcgttcccga cccctccgcc ctggctgaag ccaggggagc tggagcgcag
     3361 cgcgtccgct gcagctcatg acagagatag agatgtagat aaacgagact catctgttag
     3421 taaagatgac aaagaaaggg aaagcgtcga gaagagacac tccagccacc cttcaccagc
     3481 acctgtcctc ccggtgaatg ccctgggaca tacccgcagc tccactgaac agatccgggc
     3541 tcatctgaac actgaggctc gggagaagga caaacccaaa gagagggaga gagaccactc
     3601 ggaatcccgc aaggacctgg ccgccgacga gcacaaggcg aaagagggcc acctgcccga
     3661 gaaggacggg cacggccacg aggggcgcgc cgcgggcgaa gaggccaagc agctggcccg
     3721 ggtgccgtct ccctacgtgc ggaccccggt ggtggagagt gccaggccca acagcacctc
     3781 gagccgggag gccgagccgc gcaagggtga gccggcctac gagaacccca agaagagctc
     3841 cgaggtcaag gtgaaggagg agcggaagga agaccatgac ctgcctccag aggccccgca
     3901 gacccaccgg gcctcggagc cgccgcctcc caactcctcg tccagcgtgc acccggggcc
     3961 cctggcctcg atgcccatga cggtgggggt gacgggcatt caccccatga acagcatcag
     4021 cagcctggac aggactcgca tgatgacccc cttcatgggc atcagccccc tcccgggcgg
     4081 agagcgcttc ccgtaccctt ctttccactg ggaccccatc cgggacccct tgagggatcc
     4141 ttaccgagaa cttgacattc accggagaga cccgctgggc agggacttcc tgctaaggaa
     4201 cgacccgctc caccggctct cgactccccg gctgtacgaa gccgaccgct ccttcaggga
     4261 ccgggagcct cacgactaca gccaccacca ccaccaccac caccacccgc tgtctgtgga
     4321 ccctcggcgg gagcacgagc ggggaggcca cctggacgag cgggagcgct tgcacatgct
     4381 cagagaagac tacgagcaca cgcggctcca ctccgtgcac cccgcctccc tcgacggaca
     4441 cctcccccac cccagcctca tcaccccggg actccccagc atgcactatc cccgcatcag
     4501 ccccaccgcg ggcaaccaga acggactcct caacaagacc cctccgacag cagcgctgag
     4561 cgcacctccc ccgctcatct ccacgctggg gggccgcccg gtctctccca gaaggacgac
     4621 tcctctgtcc gcagagataa gggagaggcc cccttcccac acgctgaagg atatcgaggc
     4681 ccgataactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     4741 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4801 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4861 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4921 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     4981 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5401 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     5461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5521 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5581 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5701 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5761 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     5821 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5881 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5941 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6001 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6061 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6121 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6181 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6241 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6301 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6361 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     6421 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     6481 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6541 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6601 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     6661 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     6721 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6781 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6901 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
     7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9121 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggt
//
