LOCUS       62056_23160        4946 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..792
                     /codon_start=1
                     /label=EBFP
                     /note="enhanced blue variant of GFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             802..828
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     terminator      861..1048
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            1123..2479
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2584..2602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2860..3448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3622..4479)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4480..4584)
                     /label=AmpR promoter
     promoter        4930..4946
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
       61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccctg acccacggcg tgcagtgctt cagccgctac
      301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag
      841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
      901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
     1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga
     1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
     1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
     1201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
     1261 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
     1321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
     1381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
     1441 cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc
     1501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1561 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     1621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     1741 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc
     1801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     1861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     1921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     1981 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
     2041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     2101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     2161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     2221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     2281 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     2341 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     2401 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     2461 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag
     2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
     2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
     2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     4921 acacatacga tttaggtgac actata
//
