LOCUS       pYES2.        5856 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector for galactose-inducible expression of 
            proteins in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..600
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      609..856
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1097..1685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1859..2716)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2815..3615)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3616..3836)
                     /label=URA3 promoter
     rep_origin      complement(4435..5315)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5326..5839)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
      541 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
      601 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
      661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
      721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
      781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
      841 gaaggcttta atttgcggcc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg
     1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1501 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1921 cctgactccc cgtcgtgtag ataactacga tacgggagcg cttaccatct ggccccagtg
     1981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2161 ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt tggtatggct tcattcagct
     2221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2461 gcccggcgtc aatacgggat aatagtgtat cacatagcag aactttaaaa gtgctcatca
     2521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2701 aatgttgaat actcatactc ttcctttttc aatgggtaat aactgatata attaaattga
     2761 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
     2821 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
     2881 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
     2941 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
     3001 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
     3061 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
     3121 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
     3181 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
     3241 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
     3301 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     3361 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     3421 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     3481 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     3541 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     3601 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
     3661 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
     3721 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     3781 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc
     3841 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac
     3901 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
     3961 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
     4021 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
     4081 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
     4141 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
     4201 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     4261 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     4321 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     4381 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     4441 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     4501 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     4561 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt
     4621 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     4681 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     4741 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     4801 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     4861 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     4921 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     4981 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     5041 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     5101 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     5161 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     5221 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     5281 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat
     5341 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
     5401 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa cgaatagccc
     5461 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     5521 ccagtttcca acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5581 agggtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     5641 tcgaggtgcc gtaaagcagt aaatcggaag ggtaaacgga tgcccccatt tagagcttga
     5701 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggggct
     5761 agggcggtgg gaagtgtagg ggtcacgctg ggcgtaacca ccacacccgc cgcgcttaat
     5821 ggggcgctac agggcgcgtg gggatgatcc actagt
//
