LOCUS       V012745                 5009 bp    DNA     circular SYN 11-SEP-2021
DEFINITION  Exported.
ACCESSION   V012745
VERSION     V012745
KEYWORDS    pYD1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             534..794
                     /gene="AGA2"
                     /label="A-agglutinin-binding subunit"
                     /note="A-agglutinin-binding subunit from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P32781"
     CDS             858..887
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             891..914
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
     CDS             993..1034
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             1044..1061
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(1436..2107)
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     promoter        complement(2108..2209)
                     /label="TRP1 promoter"
     misc_feature    complement(2286..2789)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2826..2930
                     /label="AmpR promoter"
     CDS             2931..3788
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3962..4550
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4838..4859
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4874..4904
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4912..4928
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4936..4952
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4973..4991
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagctaattc tacttcatac attttcaatt aagatgcagt
      541 tacttcgctg tttttcaata ttttctgtta ttgcttcagt tttagcacag gaactgacaa
      601 ctatatgcga gcaaatcccc tcaccaactt tagaatcgac gccgtactct ttgtcaacga
      661 ctactatttt ggccaacggg aaggcaatgc aaggagtttt tgaatattac aaatcagtaa
      721 cgtttgtcag taattgcggt tctcacccct caacaactag caaaggcagc cccataaaca
      781 cacagtatgt ttttaagctt ctgcaggcta gtggtggtgg tggttctggt ggtggtggtt
      841 ctggtggtgg tggttctgct agcatgactg gtggacagca aatgggtcgg gatctgtacg
      901 acgatgacga taaggtacca ggatccagtg tggtggaatt ctgcagatat ccagcacagt
      961 ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac cctctcctcg
     1021 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     1081 ctgataacaa cagtgtagat gtaacaaaat cgactttgtt cccactgtac ttttagctcg
     1141 tacaaaatac aatatacttt tcatttctcc gtaaacaaca tgttttccca tgtaatatcc
     1201 ttttctattt ttcgttccgt taccaacttt acacatactt tatatagcta ttcacttcta
     1261 tacactaaaa aactaagaca attttaattt tgctgcctgc catatttcaa tttgttataa
     1321 attcctataa tttatcctat tagtagctaa aaaaagatga atgtgaatcg aatcctaaga
     1381 gaattgggca agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct
     1441 tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac
     1501 ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg
     1561 gcgtcagtcc accagctaac ataaaatgta agctctcggg gctctcttgc cttccaaccc
     1621 agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca
     1681 agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg
     1741 gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact
     1801 catctccgtg cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct
     1861 ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat
     1921 atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat
     1981 tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct
     2041 ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa
     2101 cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc
     2161 accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaattt cttgaagacg
     2221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2281 ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
     2341 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
     2401 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
     2461 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
     2521 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
     2581 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
     2641 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
     2701 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
     2761 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
     2821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2941 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3001 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3061 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3121 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     3181 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3241 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3301 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3361 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3421 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3481 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3541 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3601 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3661 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca
     3721 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3781 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3841 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3901 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3961 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4021 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4081 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4141 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4201 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4261 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4321 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     4381 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4441 agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4501 ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca
     4561 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4621 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4681 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4741 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt
     4861 aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga attgtgagcg
     4921 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc ggaattaacc
     4981 ctcactaaag ggaacaaaag ctggctagt
//
