LOCUS       40924_47778        6314 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1336, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6314)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..3352)
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     misc_feature    complement(3353..3460)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3465..3810)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3912..4164)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4168..4716)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4725..4908)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5043..5067)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5257..6048)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgacttata aagttcatcc ataccatcag tagaatttct agcaacagca gattcgtaaa
     2701 cagtaacctt attgtaatcc ttatcatggt taagaatttc gattctatga tcaacgaaat
     2761 ggtaatcagg caacttaaca gccttcttag ctctgtagat agtcttaaaa tcaactctat
     2821 gatgtcctcc tccttcaaga agcaacttat gcttaacatc tcccttaaga actccatctc
     2881 taacatacat tctttcagta gaagcatccc atccagtagt cttcttttgc ataacaggtc
     2941 cgttaggagg aaagttttct cccttaagat ggatttcgta gatgaaagaa tcttcttcca
     3001 tagagatatc agacttaacc ttaacgattc ccttatcttc gaaagtcata gttctttccc
     3061 aagagtatcc ttcaggaaaa gattgcttga agtagttagg gatatcatca gggtacttag
     3121 taaaagctct gtttccataa gcgaaagcag tagtaaggat atcataagaa aaaggaagag
     3181 gagctccttc cttaacttca agattaatag tattagttcc atcataaggc tttccttctc
     3241 cttctccttc aataacaaaa gcatgtccgt taacatttcc ttccatctta agtttgatct
     3301 tcatatcagg cttaataact cccatagtag tttcttctcc cttagaaacc atggtaccgg
     3361 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     3421 atccactagt tctagagcgg ccgccaccgc ggtggagctc caattgtcct ctccaaatga
     3481 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     3541 cttacgtcag tggagatgtc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     3601 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     3661 agatcttgaa tgatagcctt tcctttatcg caatgatggc atttgtagga gccaccttcc
     3721 ttttctactg tcctttcgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     3781 cccgaaatta tcctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     3841 ttgacatttt tggagtagac cagagtgtcg tgctccacca tgttaccggt cgtacgaggc
     3901 ctattaatcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     3961 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     4021 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     4081 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     4141 ttattgccaa atgtttgaac gatctcagat ctcggtgacg ggcaggaccg gacggggcgg
     4201 aaccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa
     4261 gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct
     4321 cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga
     4381 cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac
     4441 gtacacggta gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggccagcgta
     4501 ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag
     4561 acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt
     4621 gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc
     4681 acggcggatg tcggccgggc gtcgttctgg gctcatagat ccggtgcaga ttatttggat
     4741 tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag
     4801 catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca
     4861 ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg
     4921 ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg
     4981 gcgggggtca taacgtgact cccttaattc tccgctcatg atcggcgcgc cacgtgacgc
     5041 gtgtttacac cacaatatat cctgccacga tttgcccatg ggggtgggcg aagaactcca
     5101 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     5161 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     5221 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     5281 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     5341 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     5401 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     5461 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     5521 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     5581 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     5641 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     5701 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     5761 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     5821 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgaa gttcattcag
     5881 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     5941 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     6001 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     6061 tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     6121 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     6181 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     6241 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     6301 gacattcatc ccag
//
