LOCUS       40924_47758        6323 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1332, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6323)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..3361)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(3362..3469)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3474..3819)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3921..4173)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4177..4725)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4734..4917)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5052..5076)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5266..6057)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     2701 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     2761 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     2821 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     2881 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     2941 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     3001 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     3061 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     3121 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     3181 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     3241 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     3301 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     3361 tggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca
     3421 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc aattgtcctc
     3481 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     3541 gcgtcatccc ttacgtcagt ggagatgtca catcaatcca cttgctttga agacgtggtt
     3601 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     3661 tcggcagaga gatcttgaat gatagccttt cctttatcgc aatgatggca tttgtaggag
     3721 ccaccttcct tttctactgt cctttcgatg aagtgacaga tagctgggca atggaatccg
     3781 aggaggtttc ccgaaattat cctttgttga aaagtctcaa tagccctttg gtcttctgag
     3841 actgtatctt tgacattttt ggagtagacc agagtgtcgt gctccaccat gttaccggtc
     3901 gtacgaggcc tattaatccc gatctagtaa catagatgac accgcgcgcg ataatttatc
     3961 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     4021 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     4081 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     4141 taagaaactt tattgccaaa tgtttgaacg atctcagatc tcggtgacgg gcaggaccgg
     4201 acggggcgga accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc
     4261 gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat
     4321 gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc
     4381 ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg
     4441 gggggagacg tacacggtag actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg
     4501 gccagcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg
     4561 ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa
     4621 gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc
     4681 ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatagatc cggtgcagat
     4741 tatttggatt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg
     4801 tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt
     4861 gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg
     4921 gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     4981 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcggcgcgcc
     5041 acgtgacgcg tgtttacacc acaatatatc ctgccacgat ttgcccatgg gggtgggcga
     5101 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
     5161 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
     5221 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
     5281 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     5341 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     5401 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     5461 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     5521 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     5581 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     5641 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     5701 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     5761 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     5821 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgaag
     5881 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     5941 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     6001 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     6061 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     6121 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     6181 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     6241 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     6301 ccagtagctg acattcatcc cag
//
