LOCUS       40924_47753        7408 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1331, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7408)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7408)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2638..4446)
                     /label=GUS
                     /note="beta-glucuronidase"
     misc_feature    complement(4447..4554)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(4559..4904)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(5006..5258)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5262..5810)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(5819..6002)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6137..6161)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6351..7142)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgctc gcgatcattg
     2641 tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag
     2701 aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt taccgccaac
     2761 gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt caccgacgac
     2821 ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact gatactcttc
     2881 actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc cgtattcggt
     2941 gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagtacctt
     3001 ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt tcaggcacag
     3061 cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta cattgacgca
     3121 ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg cgaaatattc
     3181 ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg
     3241 gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt gctgagtttc
     3301 cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat
     3361 gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga tgccatgttc
     3421 atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc
     3481 cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga atcctttgcc
     3541 acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt tgtggttaat
     3601 caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgaagcg ggtagatatc
     3661 acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt cacccggttg
     3721 ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag ttgcaaccac
     3781 ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca cctgccagtc
     3841 aacagacgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat cgtccaccca
     3901 ggtgttcggc gtggtgtaga gcattacgct gcgatggatt ccggcatagt taaagaaatc
     3961 atggaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc ccggcgggat
     4021 agtctgccag ttcagttcgt tgttcacaca aacggtgata cgtacacttt tcccggcaat
     4081 aacatacggc gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc
     4141 ctgattattg acccacactt tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg
     4201 ctggcctgcc caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccgcata
     4261 attacgaata tctgcatcgg cgaactgatc gttaaaactg cctggcacag caattgcccg
     4321 gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt cgcgatccag
     4381 actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg
     4441 taacatggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     4501 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccaattg
     4561 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     4621 attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgaagacg
     4681 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     4741 cactgtcggc agagagatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
     4801 aggagccacc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga
     4861 atccgaggag gtttcccgaa attatccttt gttgaaaagt ctcaatagcc ctttggtctt
     4921 ctgagactgt atctttgaca tttttggagt agaccagagt gtcgtgctcc accatgttac
     4981 cggtcgtacg aggcctatta atcccgatct agtaacatag atgacaccgc gcgcgataat
     5041 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     5101 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     5161 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     5221 aatcttaaga aactttattg ccaaatgttt gaacgatctc agatctcggt gacgggcagg
     5281 accggacggg gcggaaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag
     5341 ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg
     5401 tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc
     5461 tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg
     5521 tggcgggggg agacgtacac ggtagactcg gccgtccagt cgtaggcgtt gcgtgccttc
     5581 caggggccag cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga
     5641 tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta
     5701 cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg
     5761 tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat agatccggtg
     5821 cagattattt ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg
     5881 gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga
     5941 cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac
     6001 ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt
     6061 gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct catgatcggc
     6121 gcgccacgtg acgcgtgttt acaccacaat atatcctgcc acgatttgcc catgggggtg
     6181 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     6241 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     6301 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     6361 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     6421 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     6481 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     6541 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     6601 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     6661 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     6721 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     6781 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     6841 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     6901 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     6961 tgaagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     7021 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     7081 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     7141 atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc
     7201 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     7261 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     7321 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     7381 atagcccagt agctgacatt catcccag
//
