LOCUS       40924_47738        5890 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1327, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 5890)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..3331)
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     misc_feature    complement(3338..3445)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     terminator      complement(3488..3740)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3744..4292)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4301..4484)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4619..4643)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4833..5624)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgacttgtg ccccagtttg ctagggaggt cgcagtatct ggccacagcc acctcgtgct
     2701 gctcgacgta ggtctctttg tcggcctcct tgattctttc cagtctgtgg tccacatagt
     2761 agacgccggg catcttgagg ttcttagcgg gtttcttgga tctgtatgtg gtcttgaagt
     2821 tgcagatcag gtggcccccg cccacgagct tcagggccat gtcgcttctg ccttccaggc
     2881 cgccgtcagc ggggtacagc atctcggtgt tggcctccca gccgagtgtt ttcttctgca
     2941 tcacagggcc gttggatggg aagttcaccc ctctgatctt gacgttgtag atgaggcagc
     3001 cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg tatgtggtga
     3061 ctctctccca tgtgaagccc tcagggaagg actgcttaaa gaagtcgggg atgccctggg
     3121 tgtggttgat gaaggttctg ctgccgtaca tgaagctggt agccaggatg tcgaaggcga
     3181 aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc tcgtagggct
     3241 tgccttcgcc ctcggatgtg cacttgaagt ggtggttgtt cacggtgccc tccatgtaca
     3301 gcttcatgtg catgttctcc ttaatcagct cgctcatggt accgggcccc ccctcgaggt
     3361 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     3421 agcggccgcc accgcggtgg agctccaatt ggccctatag accggtcgta cgaggcctat
     3481 taatcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     3541 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     3601 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     3661 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     3721 tgccaaatgt ttgaacgatc tcagatctcg gtgacgggca ggaccggacg gggcggaacc
     3781 ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg
     3841 gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg
     3901 tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc
     3961 agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac
     4021 acggtagact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc agcgtaggcg
     4081 atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg
     4141 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
     4201 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
     4261 cggatgtcgg ccgggcgtcg ttctgggctc atagatccgg tgcagattat ttggattgag
     4321 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     4381 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     4441 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     4501 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     4561 gggtcataac gtgactccct taattctccg ctcatgatcg gcgcgccacg tgacgcgtgt
     4621 ttacaccaca atatatcctg ccacgatttg cccatggggg tgggcgaaga actccagcat
     4681 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     4741 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     4801 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     4861 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4921 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     4981 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     5041 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     5101 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     5161 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     5221 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     5281 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     5341 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5401 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgaagttc attcagggca
     5461 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5521 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5581 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     5641 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     5701 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     5761 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     5821 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     5881 ttcatcccag
//
