LOCUS       62056_22845        6601 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(5..61)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      269..355
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      447..474
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        493..584
                     /label=AmpR promoter
     rep_origin      complement(971..1559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4413..5069)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(6221..6298)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        6528..6556
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6564..6580
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
       61 cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
      121 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
      181 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
      241 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
      301 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      361 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
      421 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
      481 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      781 tgatgagcac ttttgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1141 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     1201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1381 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gggtgggcga
     1681 agaactccag catgagatcc ccgcgctgga ggatcatcca gccattcggg gtcgttcact
     1741 ggttcccctt tctgatttct ggcatagaag aacccccgtg aactgtgtgg ttccgggggt
     1801 tgctgatttt tgcgagactt ctcgcgcaat tccctagctt aggtgaaaac accatgaaac
     1861 actagggaaa cacccatgaa acacccatta gggcagtagg gcggcttctt cgtctagggc
     1921 ttgcatttgg gcggtgatct ggtctttagc gtgtgaaagt gtgtcgtagg tggcgtgctc
     1981 aatgcactcg aacgtcacgt catttaccgg gtcacggtgg gcaaagagaa ctagtgggtt
     2041 agacattgtt ttcctcgttg tcggtggtgg tgagcttttc tagccgctcg gtaaacgcgg
     2101 cgatcatgaa ctcttggagg ttttcaccgt tctgcatgcc tgcgcgcttc atgtcctcac
     2161 gtagtgccaa aggaacgcgt gcggtgacca cgacgggctt agcctttgcc tgcgcttcta
     2221 gtgcttcgat ggtggcttgt gcctgcgctt gctgcgcctg tagtgcctgt tgagcttctt
     2281 gtagttgctg ttctagctgt gccttggttg ccatgcttta agactctagt agctttcctg
     2341 cgatatgtca tgcgcatgcg tagcaaacat tgtcctgcaa ctcattcatt atgtgcagtg
     2401 ctcctgttac tagtcgtaca tactcatatt tacctagtct gcatgcagtg catgcacatg
     2461 cagtcatgtc gtgctaatgt gtaaaacatg tacatgcaga ttgctggggg tgcagggggc
     2521 ggagccaccc tgtccatgcg gggtgtgggg cttgccccgc cggtacagac agtgagcacc
     2581 ggggcaccta gtcgcggata ccccccctag gtatcggaca cgtaaccctc ccatgtcgat
     2641 gcaaatcttt aacattgagt acgggtaagc tggcacgcat agccaagcta ggcggccacc
     2701 aaacaccact aaaaattaat agtccctaga caagacaaac ccccgtgcga gctaccaact
     2761 catatgcacg ggggccacat aacccgaagg ggtttcaatt gacaaccata gcactagcta
     2821 agacaacggg cacaacaccc gcacaaactc gcactgcgca accccgcaca acatcgggtc
     2881 taggtaacac tgagtaacac tgaaatagaa gtgaacacct ctaaggaacc gcaggtcaat
     2941 gagggttcta aggtcactcg cgctagggcg tggcgtaggc aaaacgtcat gtacaagatc
     3001 accaatagta aggctctggc ggggtgccat aggtggcgca gggacgaagc tgttgcggtg
     3061 tcctggtcgt ctaacggtgc ttcgcagttt gagggtctgc aaaactctca ctctcgctgg
     3121 gggtcacctc tggctgaatt ggaagtcatg ggcgaacgcc gcattgagct ggctattgct
     3181 actaagaatc acttggcggc gggtggcgcg ctcatgatgt ttgtgggcac tgttcgacac
     3241 aaccgctcac agtcatttgc gcaggttgaa gcgggtatta agactgcgta ctcttcgatg
     3301 gtgaaaacat ctcagtggaa gaaagaacgt gcacggtacg gggtggagca cacctatagt
     3361 gactatgagg tcacagactc ttgggcgaac ggttggcact tgcaccgcaa catgctgttg
     3421 ttcttggatc gtccactgtc tgacgatgaa ctcaaggcgt ttgaggattc catgttttcc
     3481 cgctggtctg ctggtgtggt taaggccggt atggacgcgc cactgcgtga gcacggggtc
     3541 aaacttgatc aggtgtctac ctggggtgga gacgctgcga aaatggcaac ctacctcgct
     3601 aagggcatgt ctcaggaact gactggctcc gctactaaaa ccgcgtctaa ggggtcgtac
     3661 acgccgtttc agatgttgga tatgttggcc gatcaaagcg acgccggcga ggatatggac
     3721 gctgttttgg tggctcggtg gcgtgagtat gaggttggtt ctaaaaacct gcgttcgtcc
     3781 tggtcacgtg gggctaagcg tgctttgggc attgattaca tagacgctga tgtacgtcgt
     3841 gaaatggaag aagaactgta caagctcgcc ggtctggaag caccggaacg ggtcgaatca
     3901 acccgcgttg ctgttgcttt ggtgaagccc gatgattgga aactgattca gtctgatttc
     3961 gcggttaggc agtacgttct cgattgcgtg gataaggcta aggacgtggc cgctgcgcaa
     4021 cgtgtcgcta atgaggtgct ggcaagtctg ggtgtggatt ccaccccgtg catgatcgtt
     4081 atggatgatg tggacttgga cgcggttctg cctactcatg gggacgctac taagcgtgat
     4141 ctgaatgcgg cggtgttcgc gggtaatgag cagactattc ttcgcaccca ctaaaagcgg
     4201 cataaacccc gttcgatatt ttgtgcgatg aatttatggt caatgtcgcg ggggcaaact
     4261 atgatgggtc ttgttgttgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     4321 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4381 gaagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     4441 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     4501 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     4561 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     4621 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     4681 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     4741 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     4801 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaactccgga
     4861 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     4921 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     4981 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     5041 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgtc
     5101 gaagctcggc ggatttgtcc tactcaagct gatccgacaa aatccacaca ttatcccagg
     5161 tgtccggatc ggtcaaatac gctgccagct catagaccgt atccaaagca tccggggctg
     5221 atccccggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     5281 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgtggtttgc cccagcaggc
     5341 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     5401 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     5461 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     5521 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     5581 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     5641 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     5701 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     5761 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     5821 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     5881 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     5941 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     6001 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     6061 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     6121 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     6181 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     6241 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg atggtgtcaa
     6301 cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg atccgggctt atcgactgca
     6361 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
     6421 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
     6481 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
     6541 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagaat
     6601 t
//
